SMALL, ROUND CELL TUMOR MONOCLONAL ANTIBODY REACTIVITY

儿童原始肿瘤(即经典的尤文氏肉瘤， 神经母细胞瘤、淋巴瘤和软组织肉瘤)， 在形态上难以区分。超微结构和免疫细胞化学 技术是有用的，但不是万无一失的。单抗(MoAbbs) 它们识别神经、淋巴(与人类白细胞抗原相关)和组织特异性 决定因素在区分这些实体时可能是有用的。我们有 用流式细胞荧光法研究了20多个细胞系和40多个细胞系 冰冻切片免疫细胞化学染色12株抗肿瘤单抗 找到可重现的反应性模式，这有助于可靠地 区分所有神经肿瘤和血液系统恶性肿瘤。尤因的 肉瘤类似于横纹肌肉瘤，但与 某些神经性MoAbbs。摘要周围神经上皮瘤是一种独特的肿瘤。 肉瘤和神经肿瘤之间的反应中间产物 尤因肉瘤。因此，大多数肿瘤都很容易识别，即使是在 没有任何明显的形态特征。这些 结果具有重要的诊断和治疗意义，但进一步 需要对更多的肿瘤进行研究。

Primitive childhood tumors (i.e., classically Ewing's sarcoma, neuroblastoma, lymphoma, and soft tissue sarcoma), are frequently morphologically indistinguishable. Ultrastructural and immunocytochemical techniques are useful but not infallible. Monoclonal antibodies (MoAbs) which recognize neural, lymphoid (HLA-related), and tissue-specific determinants might be useful in distinguishing these entities. We have studied more than 20 cells lines by flow cytofluorometry and more than 40 tumors by frozen section immunocytochemistry with a panel of 12 MoAbs and find reproducible patterns of reactivity which serve to reliably distinguish all neural tumors and hematopoietic malignancies. Ewing's sarcoma is similar to rhabdomyosarcoma, but shows some reactivity with certain neural MoAbs. Peripheral neuroepithelioma is a unique tumor with reactivity intermediate between sarcomas and neural tumors, not unlike Ewing's sarcoma. Thus, most of the tumors are readily recognized, even in the absence of any distinguishing morphologic characteristic. These results have important diagnostic and therapeutic implications, but further study of more tumors is required.