MICROBIAL ANTIGENS ASSOCIATED WITH SPECIFIC ADHERENCE

对放线菌菌毛粘附素的研究仍在继续 粘液菌是一种定植于牙齿表面的口腔细菌，形成牙齿 菌斑并导致牙周炎和牙周病变 疾病。放线菌的1型菌毛似乎是主要的 牙面粘附素对细菌吸附的研究 唾液处理过的羟基磷灰石。重组DNA技术已被用于 克隆编码A. 粘菌菌株T14V。这种蛋白质的相对分子质量为62,000 与单特异性抗体和单抗均有反应。 针对1型放线菌菌毛。的2型菌毛 放线菌在抗原性和功能上都不同于1型。 2菌毛解释了放线菌对乳糖敏感的共聚集 与血链球菌的菌株，因此有望促进 牙菌斑内细菌间黏附。编码该类型的基因 克隆了T14V株2个菌毛亚基，并对其进行了表达 在大肠杆菌中进行了部分纯化。除了这项研究之外 对于2型菌毛，免疫化学研究仍在继续 作为放线菌受体的链球菌碳水化合物 凝集素。血链霉菌34的受体相关多糖已被 纯化并发现以N-乙酰半乳糖胺为免疫优势 一群人。这些发现已经开始为 了解口腔微生物黏附的机制。

Studies are continuing to define the fimbrial adhesins of Actinomyces viscosus, an oral bacterium that colonizes tooth surfaces, forms dental plaque and contributes to the initiation of gingivitis and periodontal diseases. The type 1 fimbriae of actinomyces appear to be a principal adhesin for tooth surfaces as shown by studies of bacterial adsorption to saliva-treated hydroxyapatite. Recombinant DNA technology has been used to clone a structural gene that encodes the type 1 fimbrial subunit of A. viscosus strain T14V. This protein has a molecular weight of 62,000 daltons and is reactive with monospecific as well as monoclonal antibodies directed against actinomyces type 1 fimbriae. The type 2 fimbriae of actinomyces are antigenically and functionally distinct from type 1. Type 2 fimbriae account for the lactose-sensitive coaggregation of actinomyces with strains of Streptococcus sanguis and thus would be expected to promote interbacterial adherence within dental plaque. The gene encoding the type 2 fimbrial subunit of strain T14V has been cloned and the protein expressed in Eschedrichia coli has been partially purified. In addition to the study of type 2 fimbriae, immunochemical studies are continuing to define streptococcal carbohydrates that function as receptors for the actinomyces lectin. The receptor-associated polysaccharide of S. sanguis 34 has been purified and found to have N-acetylgalactosamine as the immunodominant group. These findings have begun to provide a structural basis for understanding the mechanisms of oral microbial adherence.