ROLE OF NEUROPEPTIDES IN NEUROENDOCRINE REGULATION

该项目研究了几种神经肽之间的相互关系。 (血管紧张素II、加压素、促肾上腺皮质激素释放因子、生长抑素 和物质)以及它们在神经内分泌调节中的作用。 我们开发了新的定量放射自显影方法 用显微密度法研究神经肽和相关酶的受体 在大脑和外周离散核团中的神经肽代谢 老鼠的器官。 我们发现了血管紧张素II、生长抑素、P物质、心钠素 因子和高亲和力受体定位于大鼠特定区域 大脑。 体液代谢的改变增加血管紧张素的数量 选择性脑区和脑下垂体中的受体。 血管紧张素转换酶(Kininase II)也通过 选定的大鼠脑内核团的放射自显影。这种酶似乎是 精神分裂症患者脑脊液中含量较低。

The project studies the interrelationships between several neuropeptides (angiotensin II, vasopressin, corticotropin releasing factor, somatostatin and substance) and their role in neuroendocrine regulation. We developed new quantitative autoradiographic methods with computerized microdensitometry to study receptors for neuropeptides and enzymes involved in neuropeptide metabolism in discrete nuclei of the brain and peripheral organs of the rat. We have found angiotensin II, somatostatin, substance P, atrial natriuretic factor, and high affinity receptors localized in specific areas of the rat brain. Alterations in fluid metabolism increases the number of angiotensin receptors in selective brain areas and in the pituitary gland. Angiotensin-converting enzyme (Kininase II) was also quantitated by autoradiography in selected rat brain nuclei. This enzyme appears to be low in the cerebrospinal fluid of schizophrenics.