LOCALIZATION AND CHARACTERIZATION OF BRAIN NEUROPEPTIDES

1)两种促肾上腺皮质激素释放因子(CRF)样免疫反应 已经在大鼠的大脑中被发现，并具有生化特征。一 类型在漏斗系统的下丘脑中获得，另一种在 感觉后脑区域和脊髓。CRF受体已经被 在脊髓、三叉神经区和脊髓浅层发现 强化了后一种关于慢性肾功能衰竭存在的建议 下丘脑外主要感觉区。2)辣椒素治疗新生儿 以前的研究表明，大鼠会导致原发灶的选择性退变 感觉神经元和P物质，生长抑素的耗尽， 来自中枢和中枢神经的缩胆囊素和血管活性肠肽 周围感觉神经。我们现在增加了CRF，降钙素基因相关肽 (CGRP)和甘丙素(GA)被列入辣椒素敏感感受器名单 神经肽。CGRP已被证明是一种有效的血管扩张剂，因此 似乎对血压控制很感兴趣。3) CRF、心钠素的免疫组织化学分布 CGRP、GA和黑色素浓缩激素(MCH)已在 制作大鼠脑和脑立体定位图谱。4)使用 放射免疫分析(RIA)和微穿孔技术定量 促肾上腺皮质激素释放因子、心钠素、降钙素基因相关肽和促肾上腺皮质激素释放激素样肽在约50个脑内的分布 原子核已经确定。5)使用RIA和高效液体 用高效液相色谱法测定CRF、MCH、CGRP抗血清的生化 特色化的。6)CRF和CGRP的放射自显影分布 大鼠大脑中的受体位置已经被证明。

1) Two types of corticotropin releasing factor (CRF)-like immunoreactivity have been identified in the rat brain and biochemically characterized. One type is obtained in the hypothalamus in fundibular system, the other one in the sensory hindbrain area and the spinal cord. CRF receptors have been identified in the spinal trigeminal and superficial spinal cord areas strengthening the latter suggestion of a presence of CRF in extrahypothalamic primary sensory areas. 2) Capsaicin treatment of newborn rats was previously shown to cause selective degeneration of primary sensory neurons and a depletion of substance P, somatostatin, cholecystokinin and vasoactive intestinal polypeptide from central and peripheral sensory nerves. We now add CRF, calcitonin gene related peptide (CGRP) and galanin (GA) to the list of capsaicin sensitive sensory neuropeptides. CGRP has been shown to be a potent vasodilator and thus seems to be of major interest in blood pressure control. 3) Immunohistochemical distribution of CRF, atrial natriuretic factor (ANF), CGRP, GA and melanin concentrating hormone (MCH) has been demonstrated in the rat brain and stereotaxic atlases were prepared. 4) Using radioimmunoassay (RIA) and the micropunch technique quantitative distribution of CRF, ANF, CGRP and MCH-like peptides in about 50 brain nuclei has been determined. 5) Using RIA and high performance liquid chromatography (HPLC) CRF, MCH, CGRP antisera were biochemically characterized. 6) The autoradiographic distribution of CRF and CGRP receptor sites in the rat brain have been demonstrated.