DEVELOPMENT OF BIOPHYSICAL METHODS FOR STUDY OF BIO-MACROMOLECULAR REACTIONS

为了实现急冷流动，研制了一种新的惯性驱动流动系统 反应动力学在反应机理研究中的应用 Sacroplasma Reticulum，ATP酶和其他酶系统，这些酶系统不能被 随后是光学装置。 时间分辨率优于1.5 ms， 迄今为止，使用2 - 4的反应的酸淬灭来实现 乙酸二硝基苯酯作为测试反应。 每种试剂取0.25 ml， 每个数据点需要2.5 ml，而其他仪器需要2.5 ml。 计划将其用于-40摄氏度的低温淬火， 来年 该驱动器在我们的零压降热 去年就有报道称， 压力、速度和 对一系列粘度进行的热测量提供了 支持混球理论所需的信息，即 混合主要是将线动量转化为角动量的结果 势头. 迄今为止的结果表明，对于粘度为1 厘泊小于10磅/平方英寸的压差足以 以3 M/sec的速度产生99%以上的混合，热波动仅为 正负2m摄氏度 此外，还将增加光纤传感器 在接下来的一年里， 可以做实验。

A new inertial drive flow system has been developed for doing quench flow reaction kinetics in the investigation of the mechanism of reaction of Sacroplasmic Reticulum, ATPase, and other enzyme systems which cannot be followed by optical means. A time resolution of better than 1.5 ms has been achieved to date using the acid quenching of the reaction of 2-4 dinitrophenyl acetate as the test reaction. 0.25ml of each reagent are required per data point in contrast to 2.5 ml for other instruments. Extension of its use for cryoquenching at -40 degrees C is planned for the coming year. The use of this drive in our zero pressure drop thermal stopped flow system was reported last year. Pressure, velocity, and thermal measurements underway over a series of viscosities have provided information needed for the support of the ball mixer theory, i.e. that mixing is mainly the result of changing linear momentum to angular momentum. The results to date demonstrate that for a viscosity of one centipoise a pressure differential of less that 10 psi is sufficient to produce better than 99% mixing at 3 M/sec with thermal fluctuations of only plus/minus 2m degrees C. In addition optical fiber sensors will be added in the coming year so that simultaneous thermal-optical stopped flow experiments may be done.