DEVELOPMENT OF BIOCALORIMETERS FOR SOLUTION AND CELL BIOCHEMICAL STUDIES

黑色金刚石涂层聚丙烯流动池得到进一步改进 以消除长期使用后出现的微小泄漏。 树林 金属封装改善了系统的热性能。 一 安装在反应区表面的小型电加热器 允许校准和热容量测量。 安装 目前，已实现了完整的计算机控制、数据收集和分析。 正在进行中 目前正在使用该方法探索几种生物化学反应。 仪器 这些包括网格蛋白组装、tRNA、-转移酶反应 以及与各种底物的ATCase反应。 该项目的意义在于发现新的 热反应所揭示的反应途径， 对生物反应进行基本的热力学测量， 其它检测系统也是可用的。 此外，直接测量 反应焓的一个更精确的方法比determina- 用范特霍夫法，即测量反应 不同温度下的平衡。 原因是， 温度范围，4摄氏度到40摄氏度对于一个 焓的准确测定。 从而预测变化 反应平衡，一个非常重要的量，例如，药物 在代谢中，直接量热测量是更优选的。

The black diamond coated polypropylene flow cell has been further improved to eliminate microscopic leaks that develop after extended use. Woods metal encapsulating has improved the thermal properties of the system. A small electrical heater installed on the surface of the reaction region permits calibration and heat capacity measurements. The installation of complete computer control, data collection, and analysis is presently underway. Several biochemical reactions are presently being explored using the instrument. These include clatherin assembly, tRNA,-transferase reactions and ATCase reactions with various substrates. The significance of the project lies in the possibility of detecting new reaction pathways as revealed by the thermal reactions and in the ability to make basic thermodynamic measurements on biological reactions where no other detection system is available. In addition, the direct measurement of the reaction enthalpy in a much more precise method than the determina- tion by using the Van't Hoff method, i.e. measuring of the reaction equilibrium at different temperatures. The reason for this is that the range of temperature, 4 degrees C to 40 degrees C is too small for an accurate determination of the enthalpy. Thus to predict the change in reaction equilibrium, a very important quantity, in for example, drug metabolism, a direct calorimetric measurement is much to be preferred.