DEVELOPMENT OF AN EMBRYO CRYOPRESERVATION PROGRAM IN LABORATORY ANIMALS

该计划的目标是促进美国国立卫生研究院的管理 开发利用动物遗传资源(NIH AGR) 胚胎采集、培养、冷冻和移植技术 超低温储存遗传资源，提高繁殖潜力。胚胎 在含有冷冻保护剂的介质中缓慢冷冻，以允许足够的 对胚胎进行脱水，以确保解冻后的存活。在稀释之后 冷冻保护剂解冻后，对胚胎进行体外培养以评估 发展。胚胎被收集并保持在冷冻状态，直到 重新衍生种群和品系所需的。这减少了基因漂移。 并用来保护活着的殖民地免受灾难。发展中的 这项技术允许胚胎银行长期储存和 随后在遗传学家SAS的指导下进行了重新推导。 同时进行的比较研究旨在确定 修改方法，以加强女性对 诱导排卵方案和提高解冻后胚胎的存活率。

The objective of this program is to facilitate the management of the NIH Animal Genetic Resource (NIH AGR) through development and utilization of embryo collection, culture, freezing and transplantation as techniques for cryobanking genetic stock and improving reproductive potential. Embryos are frozen slowly in medium containing cryoprotectant to allow sufficient dehydration of embryos to insure post-thaw survival. Following dilution of cryoprotectant after thawing, embryos are cultured to assess in vitro development. Embryos are collected and maintained in a frozen state until needed for rederivation of stocks and strains. This reduces genetic drift and serves to safeguard live colonies from catastrophe. Development of this technology allows banking of embryos for long-term storage and subsequent rederivation under the direction of the Geneticist, SAS. Concurrent comparative research studies are designed to identify modifications in methodology which will enhance female response to ovulation induction regimens and improve post-thaw embryo viability.