IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

这项工作的目标一直是了解 自体和外源抗原肽与MHC-I类分子的相互作用 分子通过详细的动力学和平衡结合方法。这些 研究使我们能够理解潜在的生化规律 管理多肽/蛋白质的相互作用以及MHC分子如何结合 细胞内和细胞表面的多肽。 在过去的一年里，我们进一步发展了定量分析，并 检测了一些多肽/MHC以及几种多肽抗体 互动。了解这些过程对生物化学和 生物物理水平不仅为理解MHC如何 分子结合和选择自身和抗原肽，而且还提供 为双方提供合理设计多肽类似物的机会 免疫接种和自身免疫性疾病的干预。

The objective of this work has been to understand the details of the interaction of self and foreign antigenic peptides with the MHC class I molecule by detailed kinetic and equilibrium binding methods. These studies permit us to understand the underlying biochemical rules that govern peptide/protein interactions, as well as how MHC molecules bind peptides both in intracellular compartments as well as the cell surface. In the past year we have further developed quantitative assays and have examined a number of peptide/MHC as well as several peptide antibody interactions. Understanding these processes on a biochemical and biophysical level provides a basis not only for understanding how MHC molecules bind and select self and antigenic peptides, but also offers an opportunity for rational design of peptide analogs for both immunization and for intervention in autoimmune disease.