DETECTION AND CHARACTERIZATION OF PROTEIN AND DNA ADDUCTS BY MASS SPECTROMETRY

该项目既涉及利用，又涉及可持续发展， 色谱、电泳和最先进的质谱技术的集成 生物分析鉴定所需的光谱方法， 结构解析研究，以揭示详细的分子 本计划项目中异生物质毒性和致癌性的性质。 这是技术上可行的第一次获得完整的看法， DNA和蛋白质加合在相同的组织中， 相关剂量。 这些新信息将提供重要的见解， 对制定现实可靠的方法至关重要， 风险评估 到目前为止，该项目已经提供了第一个 令人兴奋的，开创性的实验，这些实验清楚地表明， 协同使用AMS检测、凝胶电泳 先进的生物质谱分析方法将提供 大分子靶蛋白的身份从环境现实 体内暴露剂量。 这些信息将直接与 DNA加合物在相同的组织，从而提供了一个全面的看法， 人类接触异生物质后改变的细胞结构 超级基金网站。

This project involves both the utilization and continued development and integration of chromatographic, electrophoretic, and the most advanced mass spectrometric methods required in bioanalytical identification and structural elucidation studies necessary to reveal the detailed molecular nature of xenobiotic toxicity and carcinogenesis in this Program project. It is technically feasible for the first time to obtain a complete view of both DNA and protein adduction in the same tissues at environmentally relevant doses. This new information will provide crucial insight of fundamental importance to formulation of realistic and reliable methods of risk assessment. To date this program project has provided the first exciting, pioneering experiments which establish clearly that the collaborative use of the combination of AMS detection, gel electrophoresis and advanced methods of biological mass spectrometry will yield information on macromolecular target protein identity from environmentally realistic exposure doses in vivo. This information will be directly correlated with DNA adducts in the same tissues and thus provide a comprehensive view of the altered cell machinery from xenobiotic exposure to humans from Superfund sites.