STRUCTURE OF RAC I PROTEIN: A RAS-LIKE SMALL G PROTEIN

我们正在研究Rac-1的NMR鉴定和动力学， 这是一种Ras样的小G蛋白（约 21 kD）。 具有 越来越多的人对细胞骨架的调节感兴趣， 它也是NADH氧化酶系统的重要组成部分， 产生超氧化物可能是信使 部分数量众多， 这种蛋白质的完全标记的样品，包括部分氘代的 完全13 C/1 'N蛋白质。 几个2D，两个3D， 与Chris Turner合作在750 MHz系统上进行的实验 用这种13 C/1 'N标记的蛋白质样品进行。 而 该项目是在雷德菲尔德实验室开始的，经双方同意， 转移到了坎贝尔实验室 新的样本目前正在 准备，并将获得额外的750 MHz光谱。

We are working on the NMR identification and dynamics of Rac-1, which is a Ras-like small G protein (approx. 21 kD). It is of growing interest being involved in regulation of the cytoskeleton, and it is also an essential component of the NADH oxidase system which produces superoxide, possibly as a messenger. Numerous partly, and fully, labeled samples of this protein including a partly deuterated fully 13C/1 'N protein were produced. Several 2D, and two 3D, experiments on the 750 MHz system in collaboration with Chris Turner were run with this 13C/1 'N-labelled protein sample. While the project was begun in the Redfield lab, by mutual agreement it has been transferred to the Campbell lab. New samples are currently being prepared, and additional 750 MHz spectra will be obtained.