SENSITIVE ENHANCEMENT OF MALDI TOF MS

我们已经比较了我们的内部构造的性能 延迟提取线性MALDI-TOF仪器与我们新获得的 商用PerSeptive STR MALDI-TOF仪器， 灵敏度和质谱质量。 在线性模式下，我们的内部 构造的工具似乎是大约一个订单的 比商业仪器更灵敏的幅度（I finale 肽的量给出了具有过量信噪比的信号 在内部构造的装置中为100：1）。 商业 仪器给出了非常高质量的光谱为~ 10 fmol量的 在反射模式下的肽。 我们继续实施改进措施 到我们相信最终会导致 以进一步提高性能。

We have compared the performance of our in-house constructed delayed-extraction linear MALDI-TOF instrument with our newly acquired commercial PerSeptive STR MALDI-TOF instrument with respect to sensitivity and mass spectrum quality. In linear mode, our in-house constructed instrument appears to be approximately one order of magnitude more sensitive than the commercial instrument (I finale amounts of peptide give signals with signal-to-noise ratios in excess of 100: 1 in the in-house constructed device). The commercial instrument gives very high quality spectra for -10fmol amounts of peptide in the reflecting mode. We continue to implement improvements to the commercial instrumentation that we believe will ultimately lead to further enhancements in performance.