INFLUENCE OF MATRIX SOLUTION CONDITIONS ON MALDI-MS OF PEPTIDES & PROTEINS

样品基质制备程序显示出极大的 影响基质辅助激光器的质量 肽和蛋白质的解吸/电离（MALDI）质谱。 特别是，在以下情况下，观察到显著的群体歧视效应： 使用基质4-羟基-？-氰肉桂酸配合物分析 肽和蛋白质的混合物。 歧视效应是 发现强烈依赖于样品基质溶液 组成、pH和样品-基质共结晶的速率 都长大了 这些发现表明， 进行和解释生物样品的MALDI分析。 结果还表明，存在一种类似色谱的 样品基质制备过程中的行为， 用于优化分析。 目前的工作描述了 复杂混合物的大部分成分 可以成功地测量肽和蛋白质。 一篇论文 描述这些结果的文献（S.L.科恩和B.T.查特， Anal. 68（1996）31-37）。我们继续调查 不同样品制备程序对 MALDI质谱中的信息含量，并继续使 具有实际重要性的发现

Sample-matrix preparation procedures are shown to greatly influence the quality of the matrix-assisted laser desorption/ionization (MALDI) mass spectra of peptides and proteins. In particular, dramatic mass discrimination effects are observed when using the matrix 4-hydroxy-?-cyanocinnamic acids for analyzing complex mixtures of peptides and proteins. The discrimination effects are found to be strongly dependent on the sample-matrix solution composition, pH, and the rates at which the sample-matrix co-crystals are grown. These findings demonstrate the need to exercise great care in performing and interpreting MALDI analysis of biological samples. The results also indicate that there is a chromatographic-like behavior in the sample-matrix preparation procedures that can be exploited to optimize the analysis. The present work describes the conditions under which the majority of components of a complex mixture of peptides and proteins can be successfully measured. A paper describing these results was published (S.L. Cohen and B.T. Chait, Anal. Chem. 68 (1996) 31-37.) We continue to investigate the influence of different sample preparation procedures on the information content in MALDI mass spectra and continue to make findings of practical importance