ORGANIC SECONDARY ION IMAGING

描述：（申请人摘要）本研究的目的是 二次离子探针-离子阱质谱法的发展 确定目标有机物的空间和组成分布， 生物组织中的化合物。 当前的特定目标 研究期间发现， 和新生大鼠。 为了完成这些分析， (SI)微探针将与离子阱集成，以创建西姆斯/MS 微探针 离子阱的添加将允许高灵敏度， 测量m/z 600-1000的离子;这种增强的灵敏度是必不可少的 到绘制甘油磷脂。 该仪器使之能够 使用精细的离子发生器从样品上的特定位置产生离子， 富勒烯初级离子的聚焦束。 富勒烯初级离子的用途 使其能够持续侵蚀样品表面，而不会导致 样品的化学变化，至少在连续的西姆斯中很明显 谱 这一功能将使开发不仅图像成为可能 基于单一成分的分布，但不同成分的分布图 可以确定相关化合物的分布。 地图基于 成年大鼠脑的磷酸胆碱二次离子发射将是 最初开发，因为类似的地图已经使用三重 四极杆质谱，和磷脂酰胆碱的分布， 成年大鼠是固定的。

DESCRIPTION: (applicant's abstract) The objective of this research is development of secondary ion probe - ion trap mass spectrometry for determining the spatial and compositional distribution of targeted organic compounds in biological tissue. The particular targets of the current research period are glcycerophospholipids distributed in the brains of adult and neonatal rats. To accomplish these analyses, a secondary ionization (SI) microprobe will be integrated with an ion trap to create a SIMS/MS microprobe. The ion trap addition will permit high sensitivity in the measurement of ions of m/z 600-1000; this enhanced sensitivity is essential to mapping glycerophospholipids. This instrument makes it possible to generate ions from a particular location on the sample using a finely focused beam of the fullerene primary ions. Use of fullerene primary ions makes it possible to continually erode the sample surface without causing chemical alterations of the sample, at least as evident in successive SIMS spectra. This feature will make it possible to develop not only images based on the distribution of a single constituent, but maps of the different distributions of related compounds can be determined. Maps based upon phosphocholine secondary ion emission from adult rat brains will be developed initially, since similar maps have been generated using triple quadrupole mass spectrometry, and the distribution of phosphatidylcholine in adult rats is fixed.