Labelled IMS TAG Proteins for Quantitative Mass Spectrometry Imaging

本项目旨在开发生物组织中蛋白质定量质谱成像（MSI）的方法。将使用两种非动物模型，它们都包括由人类细胞系制造的多细胞三维（3D）组织结构；该方法的成功开发将提供一种替代体内和离体测试的方法，使用动物模型来代表人类肿瘤和皮肤。与采用免疫组织化学和免疫荧光的传统技术相比，该开发的方法也将适用于动物组织，其中成像和量化多种非标记蛋白质的能力有可能减少许多程序中使用的动物数量。

This project aims to develop methodology for the quantitative Mass Spectrometry Imaging (MSI) of proteins in biological tissue. Two non-animal models will be used both of which comprise multi-cellular, three-dimensional (3D) tissue constructs fabricated from human cell lines; a novel type of tumour spheroid model developed in our laboratory and a commercial 3D human skin construct Successful development of the methodology will present an alternative to both in vivo and ex vivo testing using animal models to represent human tumours and skin. The developed methodology will also be applicable to animal tissue where the ability to image and quantify multiple non-labelled proteins has the potential to reduce the number of animals used in many procedures, in comparison to conventional techniques employing immunohistochemistry, and immunoflouresence.