HIGH PERFORMANCE METAL CHELATE INTERACTION CHROMATOGRAPHY

一种二亚乙基三胺功能固定相， 过渡金属离子，开发和评价用于HPLC 分居 开发了一个经验保留表达式， 色谱保留：a）酸性的静电相互作用 磺酰胺官能团与阳离子固定相，B）仲 金属-配体络合物，包括杂环芳族 溶质的取代基，和c）相对的 在移动的相中的疏水溶质。 保留率与 与有机改性剂和缓冲液的浓度成比例， 甲醇有效地使静电相互作用最小化， 允许基于金属-配体相互作用的保留。 一份基于 这项研究正在进行中。

A diethylenetriamine functional stationary phase, capable of complexing transition metal ions, was developed and evaluated for use in HPLC separations. An empirical retention expression was developed that related chromatographic retention to: a) electrostatic interaction of the acidic sulfonamide function with the cationic stationary phase, b) secondary metal-ligand complexations involving the heterocyclic aromatic substituents of the solutes and c) solubilization of the relatively hydrophobic solutes in the mobile phase. Retentions were inversely proportional to concentrations of organic modifier and buffer and 50% methanol was effective in minimizing electrostatic interactions while allowing retention based on metal-ligand interactions. A manuscript based on this study is in progress.