PRODUCTION OF ANTIBODIES SPECIFIC FOR SINGLE AIRWAY MUCIN GENE PRODUCTS

工作总结：呼吸道粘蛋白高分泌与 以呼吸道炎症为特征的各种疾病，如 哮喘、慢性支气管炎和囊性纤维化。在这些疾病中 在某些国家，呼吸道分泌物过多是发病的一个原因。人的呼吸道 上皮细胞至少表达7个粘蛋白基因。相对的 这些基因产物中的每一个对呼吸道高分泌的贡献是 目前尚不清楚。为了测量个体的分泌产物 基因在体外和呼吸道分泌物中，融合蛋白包含 呼吸道表达的粘蛋白基因独特的非糖基化表位 已经被生产并用于提高针对个体的抗血清 基因产品。创建酶联免疫分析方法将允许 体外培养上清液中粘蛋白基因特异性产物的检测 实验或在呼吸道分泌物中。

Summary of Work: Airway mucin hypersecretion is associated with a variety of diseases characterized by airway inflammation, such as asthma, chronic bronchitis, and cystic fibrosis. In these disease states, excessive airway secretion is a cause of morbidity. Human airway epithelial cells express at least 7 mucin genes. The relative contribution of each of these gene products to airway hypersecretion is unknown at present. In order to measure secreted products of individual genes in vitro and in airway secretions, fusion proteins containing unique nonglycosylated epitopes from mucin genes expressed in the airway have been produced and used to raise antisera specific for individual gene products. Creation of enzyme-linked immunoassays will allow for the assay of mucin gene-specific products in culture media from in vitro experiments or in airway secretions.