ROLE OF ADHESION MOLECULES IN MAST CELL AND BASOPHILS FUNCTION

炎症反应的特点是积聚了 损伤部位的细胞外基质蛋白。肥大细胞和 嗜碱性细胞在这种炎症部位聚集，并通过以下方式发挥作用 释放了一系列调解人。嗜碱性粒细胞和肥大细胞有表面 参与与这些细胞结合的黏附受体 其他细胞或细胞外基质。这种整合素介导的 细胞与纤维连接蛋白的黏附刺激细胞扩散并生成 细胞内信号。之前我们观察到RBL-2H3细胞结合 通过整合素受体到涂有纤维连接蛋白的表面；这 导致细胞骨架的改变，细胞的铺展和 颗粒重新分布到细胞的外围。这些 变化导致几种蛋白质的酪氨酸磷酸化 包括粘着斑激酶pp125FAK(FAK)和增强型 细胞的分泌物。FAK是一种广泛表达的蛋白酪氨酸激酶 它与整合素和几种蛋白质定位在细胞内位置 细胞与细胞外基质接触的地方。FAK还 酪氨酸被磷酸化，以响应各种 受体包括FcepsilonRI和T细胞上的抗原受体。在……里面 在今年的研究中，我们继续探索 黏附和免疫受体产生的细胞内信号 激活。

The inflammatory response is characterized by the accumulation of extracellular matrix proteins at sites of injury. Mast cells and basophils accumulate at such inflammatory sites and play a role by releasing an array of mediators. Basophils and mast cells have surface adhesion receptors that are involved in the binding of these cells to other cells or to the extracellular matrix. Such integrin mediated adherence of cells to fibronectin stimulates cell spreading and generates intracellular signals. Previously we observed that the RBL-2H3 cells bind through integrin receptors to surfaces coated with fibronectin; this results in changes in the cytoskeleton, cell spreading and a redistribution of the granules to the periphery of the cells. These changes result in the tyrosine phosphorylation of several proteins including the focal adhesion kinase, pp125FAK (FAK) and in enhanced secretion by the cells. FAK is a widely expressed protein tyrosine kinase that localizes with integrins and several proteins to intracellular sites where cells are in contact with the extracellular matrix. FAK also becomes tyrosine phosphorylated in response to the activation of various receptors including FcepsilonRI and antigen receptors on T-cells. In studies during this year we have continued exploring the interaction of intracellular signals generated by adhesion and immune receptor activation.