CEREBRAL ISCHEMIA--NEUROTRANSMITTERS, METABOLISM, AND THERAPY

我们之前的研究表明，年轻人的相对抵抗力 沙土鼠可能与神经功能有关。另一方面， 有关热休克蛋白HSP27发挥保护作用的报道 对抗神经元损伤。为了进一步阐明，这些机制 对观察到的与年龄相关的脑部易感性负责 我们研究了缺血对热休克蛋白72表达的影响。 年轻人海马区转录和翻译水平的研究 和成年沙鼠。在ALL中观察到HSP在RNA中的表达 海马区在复流后3小时内达到最大，到8小时达到最大。 幼年沙鼠和成年沙鼠的回流时间均为数小时。然而，一大堆 再灌流1小时后，原位杂交强度增强。 幼年动物的海马体比成年动物的海马体年轻。进步者 热休克蛋白72在不同区域的表达均下降。 除CA1外，其余为海马区。48h时，持续表达的mRNA 在幼年沙土鼠中，CA1的表达比成年沙鼠更为显著。…的出现 HSP72蛋白的检测时间晚于mRNA的检测时间。 最显著的不同是在CA1神经元上，它显示出更多的 HSP72蛋白在幼年的显著积聚 成年动物。目前的实验评估了基因的表达。 结构性热休克72蛋白与海马区存活 CA1神经元。数据表明HSC 72mRNA也有类似的模式 HSP72mRNA在幼年和成年沙土鼠中的表达。这个 HSP蛋白积累越明显，与更多的 青壮年大鼠海马CA1区神经元存活情况。这些发现 支持我们之前的假设，即对不成熟的内源性容忍 神经元可能与较高的转录活性有关。

Our previous studies suggested that the relative resistance of young gerbils may be attributed to neuronal function. On the other hand reports implicated of heat shock protein HSP27 to play a protective role against neuronal injury. To elucidate further, the mechanisms responsible for the observed age dependent susceptibility to brain ischemia, we investigated the effect of ischemia on HSP 72 expression, a transcriptional and translational level in the hippocampus of young and adult gerbils. The HSP in RNA expression was observed in all hippocampal areas within 3 hours of reflow, reaching the maximum by 8 hours of reflow in both young and adult gerbils. However, a much stronger in situ hybridization was observed at 1 hour of reperfusion in the hippocampus of young than that seen in adult animals. A progressive decrease in HSP 72 mRNA expression was seen in various areas of hippocampus except CA1. At 48 hours, the persisting expressions of mRNA in CA1 was more marked in young than adult gerbils. The appearance of HSP 72 protein was detected at a later time than that observed for mRNA. The most striking difference was seen in CA1 neurons which showed a more marked accumulation of HSP 72 protein in young than that observed in adult animals. Present experiments evaluated mRNA expression of constitutive heat shock 72 (HSC 72) protein and survival of hippocampal CA1 neurons. The data indicate a similar pattern of HSC 72 mRNA expression as that seen for HSP 72 mRNA in young and adult gerbils. The more marked accumulation of HSP protein is associated with greater survival of hippocampal CA1 neurons in young than adult. These findings support our previous postulation that endogenous tolerance of immature neurons may be related to higher transcriptional activity.