MOLECULAR, BIOLOGICAL, IMMUNOCHEMICAL CHYMOTRYPSIN STUDIES IN BRAIN & PERIPHERY

近年来，这方面的研究引起了人们极大的关注 蛋白水解酶，因为蛋白水解酶抑制剂已被证明有效 延长艾滋病患者的生命。我们一直在研究 凝乳酶，一种以前被认为是 仅在胰腺中合成。我们已经产生了一种兔抗血清 人类凝乳酶，并发现凝乳酶存在于 胃内绒毛和上皮细胞中的内分泌样细胞 肠子的部分。通过核糖核酸酶保护实验，我们还检测到 大鼠胃、十二指肠、卵巢和脾中凝乳酶mRNA的表达。我们 在大鼠和人脑中也发现了胰凝乳酶的mRNA。 电泳法和免疫印迹法检测到胰凝乳酶条带。 胃、十二指肠和脑。一种有效的蛋白酶的合成，如 胰凝乳素在胰腺以外的组织中显著存在，提示 在这些组织中潜在的生理和/或病理作用。

In recent times there has been a great focus of attention on the study of proteases, since protease inhibitors have been shown to be effective in extending the life of people with AIDS. We have been studying chymotrypsin, a protease enzyme that was previously believed to be synthesized only in the pancreas. We have generated a rabbit antiserum to human chymotrypsin and have found chymotrypsin to be present in endocrine-like cells of the villi in the stomach and in epithelial cells of the intestine. By a ribonuclease protection assay we also detected chymotrypsin mRNA in the rat stomach, duodenum, ovary and spleen. We have also identified chymotrypsin mRNA in the rat and human brain. Electrophoresis and immunoblotting revealed chymotrypsin bands in the stomach, duodenum and brain. Synthesis of a potent protease such as chymotrypsin in tissues other than pancreas is significant, suggesting a potential physiological and/or pathological role in these tissues.