DEVELOPMENT OF RAMAN SPECTROSCOPY FOR IN VIVO DIAGNOSIS OF ATHEROSCLEROSIS

进行了临床研究，所有患者均接受了 由一名内镜医师（SS）进行胃镜检查（EGD）。 活检和光谱检查部位测量为与 门牙 光学探头穿过附件 内窥镜的通道，并放置成与 组织. 从每个样品中收集三到四个反射光谱， 活检前检查部位。 在四个象限中重复该序列， 每层每隔两厘米 粘膜 每例患者平均评价12个部位。 每个 活检由专家病理学家评价，并表征为 包含无发育不良、低度发育不良、高度发育不良， 不确定或组织不足以诊断。 所有活检 也被表征为存在或不存在炎性 细胞 每个样本的特征是含有发育不良或 其特点是不确定的，以及一个平等的数目， 将非发育异常对照标本提交给三位专家 胃肠道病理学家进行独立和盲法分析。 收集的光谱通过该模型进行分析， 发现与病理学非常一致。

Clinical studies were performed where all patients underwent esophago gastroduodenoscopy (EGD) by a single endoscopist (SS). Biopsy and spectroscopy sites were measured as distance from the incisor teeth. The optical probe was passed through the accessory channel of the endoscope and placed into light contact with the tissue. Three to four reflectance spectra were collected from each site prior to biopsy. This sequence was repeated in four quadrants at each level at two-centimeter intervals throughout the Barrett's mucosa. An average of 12 sites was evaluated in each patient. Each biopsy was evaluated by an expert pathologist and characterized as containing no dysplasia, low grade dysplasia, high grade dysplasia, indeterminate, or tissue insufficient for diagnosis. All biopsies were also characterized for the presence or absence of inflammatory cells. Each specimen characterized as containing dysplasia or characterized as indeterminate, as well as an equal n umber of non-dysplastic control specimens were submitted to three expert gastrointestinal pathologists for independent and blinded analysis. The collected spectra were analyzed by means of the model and excellent agreement with pathology was found.