GENETIC MANAGEMENT OF ANIMAL COLONIES

意义监测和控制遗传结构 非人灵长类动物繁殖群和重建/确认 在殖民地的家谱关系是必不可少的 维持非人类灵长类动物作为研究资源。 目标 开发快速廉价的亲子鉴定方法 排除分析和估算亲缘系数和近交系数 和遗传细分和基因多样性的参数， 非人类灵长类动物 结果18个新的高度多态性 微卫星（STR）基因座，并确定了40个基因型 对这些基因座中的每一个表征不相关的雄性恒河猴。 这些基因座的基因多样性（H）估计值在0.11和0.12之间。 0.87. 其中12个信息量最大的样本的H值为0.77 （0.07），范围在0.67和0.87之间;这些位点显示， 平均每个位点7.3（2.4）个等位基因。 这12个STR基因座 用15个已报道的STR基因座对13名成员进行基因分型 试图区分已知的亲属对 通过使用这27个基因座共有的STR等位基因的数量。 不相关的对很容易从所有相关的对中区分出来。 这些结果表明，STR基因座可用于重建 在有缺失或不完整记录的群体中有未知的谱系。 这些 对60只猩猩和9只高血压猩猩的样本进行了相同的基因座筛选。 多态性位点被用来比较不同的猩猩 在博尔诺和苏门答腊的地理位置。 未来 上述STR基因座将用于筛选 其他古猿类物种，包括斑腿猴、长尾猴和吼猴， 猴子和猩猩来评估它们对遗传的有效性， 管理和恒河猴群中的恒河猴母子对 将筛选猴的所有27个高变STR基因座，以估计 恒河猴群中误产的发生率。 关键词 微卫星（STR）基因座，PCR扩增，亲权排除 分析（PEA）基金NIH Grant RR 00169 PULBLICATIONS Kanthaswamy， 美国，史密斯，D. G.微卫星多态性在亲子鉴定中的应用 恒河猴（Macaca mulatta）。 灵长 39（2）：135-145，1998.

Significance Monitoring and controlling the genetic structure of non-human primate breeding colonies and reconstructing/confirming genealogical relationships in the colonies are essential to the maintenance of non-human primates as a research resource. Objectives To develop rapid and inexpensive methods to conduct paternity exclusion analysis and estimate kinship and inbreeding coefficients and parameters of genetic subdivision and gene diversity in groups of non-human primates. Results Eighteen new highly polymorphic microsatellite (STR) loci were identified and genotypes of 40 unrelated rhesus males were characterized for each of these loci. Gene diversity (H) estimates of these loci range between 0.11 and 0.87. The value of H for the 12 most informative of these was 0.77 ((0.07) and ranged between 0.67 and 0.87; these loci exhibited, on average, 7.3 ((2.4) alleles per locus. These 12 STR loci, together with 15 STR loci previously reported were used to genotype 13 members of an extended family to attempt to differentiate known relative pairs by the number of STR alleles shared in common using these 27 loci. Unrelated pairs were easily differentiated from all related pairs. These results suggest that STR loci can be useful in reconstructing unknown pedigrees in groups with missing or incomplete records. These same loci were screened for a sample of 60 orangutans and nine highly polymorphic loci were used to compare orangutans of different geographic o rigins within Bornco and within Sumatra. Future Directions The STR loci cited above will be used to screen a variety of other cereopithecoid species including patas, vervet, and howler monkeys and orangutans to assess their effectiveness for genetic management, and mother/offspring pairs of rhesus in a group of rhesus monkeys will be screed for all 27 hypervariable STR loci to estimate the incidence of mistaken maternity in rhesus groups. KEY WORDS microsatellite (STR) loci, PCR amplification, paternity exclusion analysis (PEA) FUNDING NIH Grant RR00169 PULBLICATIONS Kanthaswamy, S., Smith, D. G. Use of microsatellite polymorphisms for paternity exclusion in rhesus macaques (Macaca mulatta). Primates 39(2):135-145, 1998.