REGULATION OF VACCINIA VIRUS GENE EXPRESSION

痘病毒编码所需的酶和因子 它们的基因组在感染的细胞质中的转录 细胞。痘苗病毒，痘病毒家族的原型成员， 提供了一种独特的系统来结合生化和遗传 基因调控和基因调控机制的研究进展 M RNA的生物合成。对牛痘病毒的研究表明， 基因被分成三个时间类--早期、中期 而且很晚了。每类基因都有一个一致的DNA启动子序列 和相应的转录因子相互作用 病毒编码的多亚单位RNA聚合酶。抄写 早期基因的系统被包装在传染性病毒中 粒子在其组装过程中，而中间体的因素 和晚期基因转录相继合成后 感染。

Poxviruses encode enzymes and factors needed for transcription of their genomes within the cytoplasm of infected cells. Vaccinia virus, the prototypic member of the poxvirus family, provides a unique system for combining biochemical and genetic approaches for investigating mechanisms of gene regulation and mRNA biosynthesis. Studies with vaccinia virus indicated that the genes are divided into three temporal classes - early, intermediate and late. Each gene class has a consensus DNA promoter sequence and corresponding transcription factors that interact with the virus-encoded multisubunit RNA polymerase. The transcription system for early genes is packaged within the infectious virus particle during its assembly, whereas the factors for intermediate and late gene transcription are synthesized successively after infection.