ARACHIDONOYL DIACYLGLYCEROL KINASE

几年前我们发现了一种新的甘油二酯 优先催化磷酸化的激酶 含有酯化花生四烯酸的二酰基甘油分子种类 酸 我们还发现，在哺乳动物中存在大量的酶， 成年狒狒的睾丸和大脑 从那时起，我们一直 探索这种酶在细胞中发挥作用的可能性， 涉及含肌醇的磷酸甘油酯的信号传导过程。 我们已经证明，这种酶存在于 突触体，也表明这种酶存在于非常 在猕猴视网膜中的浓度很高 后一个发现是 特别感兴趣，因为它提出了一种可能性， 磷脂酰肌醇依赖性细胞信号传导可能在 视觉过程 现在需要的是将酶定位在 视网膜的特定细胞和细胞膜，我们将需要 特异性抗体进行免疫化学定位。 我们 已经制备了针对酶的特定区域的抗体， 序列，并已使用这些抗体在Western印迹 实验 但我们还没有成功地利用它们来 免疫化学定位工作。 也许我们必须 在将来制备针对这种酶的单克隆抗体。

Several years ago we discovered a new form of diacylglycerol kinase that preferentially catalyses the phosphorylation of diacylglycerol molecular species that contain esterified arachidonic acid. We also showed that high amounts of the enzyme are present in the testis and brain of the mature baboon. Since then we have been exploring the possibility that the enzyme may play a role in cell signaling processes that involve inositol-containing phophoglycerides. We have shown that the enzyme is present in preparations of synaptosomes and have also shown that the enzyme is present in very high concentrations in the macaque retina. This latter finding is of particular interest because it raises the possibility that phosphatidylinositol-dependent cell signaling may play a role in the visual process. What is needed now is to localize the enzyme in specific cells and cell membranes of the retina, and we will need specific antibodies for this immunochemical localization work. We have prepared antibodies directed to specific regions of the enzyme's sequence, and have used these antibodies in Western blotting experiments. But we have not yet succeeded in using them for immunochemical localization work. It may be that we will have to prepare monoclonal antibodies to the enzyme in the future.