CAPILLARY ELECTROPHORESIS--SYNTHESIS AND MOLECULAR RECOGNITION STUDIES

这项研究将毛细管电泳(CE)作为一项技术 为了研究化合物的合成和分子识别性能 生物分子，特别是那些以无机/有机金属为前导的生物分子 试剂。 研究的具体目标是： *评估无机/有机金属试剂与 多肽和蛋白质以及其他生物分子。 *定义将CE用作在其中进行监测的微反应器的有用性 生物分子与无机/有机金属试剂和In的反应 酶催化的反应。 *开发新的合成有机金属试剂和方法 标记生物分子。 *测试CE的效用，以检查 修饰的蛋白质。 *检查无机/有机金属标记的化学反应活性 生物分子。 *探索CE在结合分析以及在非水和 混合溶剂体系。 *检查标记的生物分子的分子识别特性 与无机/有机金属试剂通过使用 羰基金属免疫分析(CMIA)技术。 这项研究将： *扩大毛细管电泳法在生物分析中的应用 应用程序、 *探索无机/有机金属试剂的化学选择性 对于生物分子， *探索蛋白质的结构和构象差异 无机/有机金属试剂， *检查标记了的生物分子的化学反应活性变化 无机/有机金属试剂，以及 *发展用于生物分子分析的羰基金属免疫分析 互动。

This research will develop capillary electrophoresis (CE) as a technique for studying the synthesis and molecular recognition properties of biomolecules in particular those label led with inorganic/organometallic reagents. The specific objectives of the research are to: * Evaluate the reactions between inorganic/organometallic reagents and peptides and proteins and other biomolecules. * Define the usefulness of using CE as a microreactor in which to monitor the reactions of biomolecules and inorganic/organometallic reagents and in enzyme-catalyzed reactions. * Develop new synthetic organometallic reagents and methods in which to label biomolecules. * Test the utility of CE to examine the physical-organic chemistry of modified proteins. * Examine the chemical reactivity of inorganic/organometallic-labelled biomolecules. * Explore applications of CE to binding assays and in non-aqueous and mixed solvent systems. * Examine the molecular recognition properties of biomolecules labelled with inorganic/organometallic reagents by the use of the carbonylmetalloimmunoassay (CMIA) technique. This research will: * Extend the usefulness of capillary electrophoresis in bioanalytical applications, * Explore the chemical selectivity of inorganic/organometallic reagents for biomolecules, * Explore the structural and conformational variances in proteins with inorganic/organometallic reagents, * Examine the changes in chemical reactivity of biomolecules labelled with inorganic/organometallic reagents, and * Develop the carbonylmetalloimmunoassay for the analysis of biomolecule interactions.