CORE--LABORATORY

实验室核心为单个项目的重组蛋白生产、蛋白纯化和这些试剂的标准质量控制提供集中资源。这是一个现有的核心单元，已经建立了重组生产Vit的所有方面的转染，选择和大规模生长的哺乳动物细胞系。依赖K的凝血因子VIIa和X.可溶性TF和位点特异性突变体在大肠杆菌或酿酒酵母中的表达是既定的常规程序，包括重新折叠和纯化到均匀性，以成功结晶这些蛋白[1,2]。血浆蛋白的纯化，以及抗体的产生和分裂的方案已经到位。因此，这个核心集中了各种项目使用的基本试剂的生产。

The laboratory core provides centralized resources for recombinant protein production, protein purification and standard quality control of these regents for the individual projects. This is an existing core unit that has established all aspects of transfection, selection and large scale growth of mammalian cell lines for the recombinant production of Vit.K dependent coagulation factors VIIa and X. Expression of soluble TF and site specific mutants in E. coli or S. cerevisiae are established routine procedures, including refolding and purification to homogeneity for successful crystallization of these proteins [1,2]. Protocols for the purification of plasma proteins, as well as, for the antibody production and fragmentation are in place. This core thus centralizes the production of essential reagents used by the various projects.