DEUTERIUM OXIDE ON FLUORESCENCE INTENSITY & LIFETIME OF DNA INTERCALATING PROBES

光谱分析与常规流动和相敏流动 用流式细胞术比较PI和EB荧光的变化 DNA和固定的中国仓鼠卵巢的结合强度和寿命 (CHO)细胞在D2O存在下与磷酸盐缓冲盐水的比较 (PBS)。PI和EB的荧光强度提高了一倍 在D2O中注意到与固定的CHO细胞结合以及5 ns的增加 在PI和EB中荧光寿命在D2O中。我们最近使用了D2O EB与人二倍体成纤维细胞结合寿命的研究 位于G1/S相变点。结果表明， 进入S相结构过程中染色质状态的变化 导致荧光强度和荧光的变化 DNA嵌入EB的寿命。

Spectroscopic analysis and conventional and phase-sensitive flow cytometry were used to compare changes in PI and EB fluorescence intensity and lifetime bound to DNA and fixed Chinese hamster ovary (CHO) cells in the presence of D2O versus phosphate buffered saline (PBS). A two-fold enhancement of fluorescence intensity of PI and EB bound to fixed-CHO cells in D2O was noted as well as a 5 ns increase in PI and EB fluorescence lifetimes in D2O. We have recently used D2O in studies on the lifetime of EB bound to human diploid fibroblasts located at the G1/S phase transition point. Results indicate that alterations chromatin states during the entry into S phase structures lead to alterations in the fluorescence intensity and fluorescence lifetime of the DNA-intercalated EB.