PURCHASE OF LCQ MASS SPECTROMETER SYSTEM

该补助金的目的是申请资助ThermoQuest Finnigan MAT LCQ十离子阱质谱仪（MS）。目前在达特茅斯的合理行驶距离内没有可用于大分子质量分析的设备。所有需要对蛋白质、肽、寡核苷酸、有机化合物和其他大分子进行质量分析的研究人员目前都被迫临时使用外部服务。LCQ-MS Deca将被整合到达特茅斯学院分子生物学（MB）核心设施中。该设施现在为75个实验室提供蛋白质微量化学、蛋白质测序、DNA测序和DNA合成。MB核心设施在一位教师主任和一位教师顾问委员会的控制下运作。一名专业经理和两名技术支持人员负责日常运营。该设施是达特茅斯研究界的共享资源，即，来自达特茅斯艺术与科学学院、达特茅斯医学院、诺里斯棉花癌症中心和位于佛蒙特州白河交汇处的退伍军人管理局医疗中心的研究人员。达特茅斯医学院和达特茅斯艺术与科学学院已经对这一点和其他核心设施作出了实质性的承诺，作为遗传学和结构生物学领域的机构范围扩展的一部分。此外，艺术与科学学院通过NIEHS/EPA超级基金计划项目支持MB核心设施，诺里斯棉花癌症中心继续通过其NCI癌症中心核心赠款提供支持。该仪器的一个主要用户组已确定由8名NIH资助的研究人员组成。这组研究人员使用分子生物学核心设施提供的其他服务，并希望在相同的服务收费基础上使用LCQ Deca MS。主要用户组目前拥有11个NIH资助的研究赠款，每年直接费用总额为280万美元。

The purpose of this grant is to request funding for a ThermoQuest Finnigan MAT LCQ Deca Ion Trap Mass Spectrometer (MS). No equipment is currently available for the mass analysis of macromolecules at or within reasonable traveling distance of Dartmouth. All investigators in need of mass analysis of proteins, peptides, oligonucleotides, organometalic compounds and other macromolecules are currently forced to use out-of-house services on an ad hoc basis. The LCQ-MS Deca will be integrated into the Dartmouth College Molecular Biology (MB) Core facility. This facility now offers protein microchemistry, protein sequencing, DNA sequencing and DNA synthesis to 75 laboratories. The MB Core Facility operates under the control of a faculty Director and a faculty Advisory Board. A professional Manager and two support technicians handle day-to-day operations. This facility is a shared resource for the Dartmouth research community, i.e., researchers from the Dartmouth College of Arts & Sciences, Dartmouth Medical School, the Norris Cotton Cancer Center and the Veterans Administration Medical Center in White River Junction, VT. The Dartmouth Medical School and the Dartmouth College of Arts & Sciences have made a substantially commitment to this and other core facilities as part of an institution-wide expansion in the fields of Genetics and Structural Biology. In addition, the College of Arts & Sciences supports the MB Core facility through an NIEHS/EPA Superfund Program Project, and the Norris Cotton Cancer Center continues its support through its NCI Cancer Center Core grant. A major users group for this instrument has been identified consisting of 8 NIH-funded investigators. This group of investigators uses the other services provided by the Molecular Biology Core Facility and expects to use the LCQ Deca MS on the same fee-for- service basis. The major users group currently holds 11 NIH funded research grants totaling $2.8 million in total direct costs annually.