NUMERICAL SIMULATION OF ILLUMINATION PROFILES FOR HIGH NA OBJECTIVES

第46届辐射研究学会年会， 肯塔基州路易斯维尔活细胞和完整组织的光学成像 非线性、同时的多光子分子激发提供了 固有的三维衍射极限分辨率 最大限度地减少光损伤和周边光漂白。多光子 激光扫描显微镜栅格扫描聚焦的高重复率 红外激光在生物体内的飞秒脉冲序列 采样并有效地检测到所产生的多光子激发 荧光。这一发展最近允许量化 细胞过程和组织结构的可视化 用传统的显微镜是有问题的或不可能的。这个 物理原理，仪器发展的方面，以及 分子荧光团的光物理特性 将讨论启用的多光子显微镜。例如 神经递质分泌、皮肤形态、药物代谢 完整的结肠，同时进行代谢和光敏剂成像 在图莫尔，椭球体展示了一系列应用 这一新形式的显微镜在开发资源上 康奈尔大学生物物理成像与光电子学(DRBIO)。 在DRBIO由NIH RR04224支持。M.G.N.是美国国立卫生研究院博士后 由NRSA 1 F32 CA 72225-02支持的研究员。

46th annual meeting of the Radiation Research Society Conference, Louisville, KY Optical imaging of living cells and intact tissues by non-linear, simultaneous multiphoton molecular excitation provides inherent three-dimensional diffraction-limited resolution while minimizing photodamage and peripheral photobleaching. The multiphoton laser scanning microscope raster-scans a focused, high repetition-rate femtosecond pulse train of infrared laser light across biological samples and efficiently detects the resulting multiphoton excited fluorescence. This development has recently allowed quantitative visualization of cellular processes and tissue architecture that had been problematic or impossible with conventional microscopies. The physical principles, aspects of instrumentation developments, and photophysical characterization of molecular fluorophores that have enabled multiphoton microscopy will be discussed. Examples such as neurotransmitter secretion, skin morphology, drug metabolism in the intact colon, and simultaneous metabolic and photosensitizer imaging in t umor spheroids demonstrate a range of applications pursued with this novel form of microscopy at the Developmental Resource for Biophysical Imaging and Opto-electronics (DRBIO) at Cornell. Supported at DRBIO by the NIH RR04224. M.G.N. is an NIH Postdoctoral Fellow supported by NRSA 1 F32 CA 72225-02.