WOMENS HEALTH ACROSS THE NATION--ENDOCRINE LAB

为了评估中年女性的内分泌变化，密歇根大学的中央配基分析卫星服务(CLASS)实验室将继续支持全国妇女健康研究(SWAN)。我们将对所有主要的生殖轴激素(即黄体生成素、卵泡刺激素、雌二醇、孕酮和睾酮)、肾上腺衰老标记物(DHEAS)、其他内分泌标记物(TSH、性激素结合球蛋白[SHBG})和新的卵巢标记物(可能使我们更精确地定义更年期过渡和绝经后的卵巢标记物(抑制素A和B、雌酮))使用最先进的自动化分析。为了继续支持每日激素研究(DHS)，CLASS将完成对黄体生成素、卵泡刺激素以及主要尿液代谢物雌二醇和孕酮、雌酮结合物和孕二醇葡萄糖醛酸苷的敏感和特异的尿液分析。我们在拟议项目期间的具体目标是：1)利用、实施和开发最先进、可靠、精确和成本效益高的分析方法，满足所有研究需要(分析物选择、检测限、性能范围)，最大限度地减少变异性，提供长期稳定性和最大限度地提高可靠性；2)纳入质量保证程序，根据严格的质量控制程序评估，将误差和变异性降至最低；3)利用和开发额外的电子数据处理和分析程序，除了降低人工成本外，最大限度地减少人工干预的需要，并几乎消除出错的可能性；4)利用样品标签和处理系统，确保标签样品和用试管识别的数据之间的连续关联，同时最大限度地保持样品的完整性不加修改的可能性；以及5)开发临时储存库。通过这种方式，我们将继续在推进母天鹅研究的科学目标方面发挥关键作用。

To assess endocrine changes in midlife women, the University of Michigan's Central Ligand Assay Satellite Services (CLASS) laboratory will continue to support the Study of Women's Health Across the Nation (SWAN). We will use state-of-the-science, automated assays for all the major reproductive axis hormones (i.e., LH, FSH, estradiol, progesterone, and testosterone), adrenal markers of aging (DHEAS), other endocrine markers (TSH, sex hormone binding globulin {SHBG}) and new ovarian markers which have the potential to allow us to hormonally define the menopausal transition and the postmenopause with greater precision (inhibin A and B, estrone). In order to continue to support the Daily Hormone Study (DHS), CLASS will complete development and utilize sensitive and specific urinary assays for LH, FSH and the principal urinary metabolites of estradiol and progesterone, estrone conjugates and pregnanediol glucuronide, respectively. Our specific goals for the proposed project period are to: 1) utilize, implement and develop state-of-the-science, reliable, precise and cost-effective analytical methods that meet all study needs (analyte selection, limits of detection, range of performance), minimize variability, provide long-term stability and maximize reliability; 2) incorporate quality assurance procedures that minimize error and variability as assessed by rigorous quality control procedures; 3) utilize and develop additional electronic data handling and analysis procedures that, in addition to reducing labor costs, minimize the need for manual intervention and nearly eliminate the possibility of errors; 4) utilize sample labeling and handling systems that ensure continuous association between a labeled sample and data identified with the tube while maximizing the likelihood that the integrity of a sample is preserved unmodified; and 5) develop a temporary storage repository. In this manner, we will continue to play a key role in the advancement of the scientific goals of the parent SWAN study.