PRODUCTION OF ANTIBODIES SPECIFIC FOR SINGLE AIRWAY MUCIN GENE PRODUCTS

呼吸道粘蛋白高分泌与 以呼吸道炎症为特征的各种疾病，如 哮喘、慢性支气管炎和囊性纤维化。在这些疾病中 在某些国家，呼吸道分泌物过多是发病的一个原因。人类 呼吸道上皮细胞至少表达7个粘蛋白基因。这个 这些基因产物对呼吸道的相对贡献 目前还不清楚过度分泌。测量分泌物 单个基因在体外和呼吸道分泌物中的产物，我们 已经产生了含有独特的非糖基化的融合蛋白 在呼吸道表达的粘蛋白基因的表位。它们被用来 提高针对单个基因产物的特异性抗血清。创建 酶联免疫分析法可用于检测 体外培养上清液中的粘蛋白基因特异性产物 实验或在呼吸道分泌物中。此外，含有 包含独特的粘蛋白基因DNA序列的插入物 已知在人类呼吸道上皮细胞中表达 创建的目的是为了创建核探针。这些核探针 将用于粘蛋白基因mRNA的定量分析。 呼吸道细胞。

Airway mucin hypersecretion is associated with a variety of diseases characterized by airway inflammation such as asthma, chronic bronchitis, and cystic fibrosis. In these disease states, excessive airway secretion is a cause of morbidity. Human airway epithelial cells express at least seven mucin genes. The relative contribution of each of these gene products to airway hypersecretion is unknown at present. To measure secreted products of individual genes in vitro and in airway secretions, we have produced fusion proteins containing unique nonglycosylated epitopes from mucin genes expressed in the airway. They are used to raise antisera specific for individual gene products. Creation of enzyme-linked immunoassays allows for the assay of mucin-gene-specific products in culture media from in vitro experiments or in airway secretions. In addition, plasmids with inserts containing unique DNA sequences of the mucin genes known to be expressed in human airway epithelial cells have been created to allow for the creation of riboprobes. These riboprobes will be used for the quantitative analysis of mucin gene mRNA in airway cells.