Cell Microarrays for High Throughput RNAi

描述(由申请人提供)：这项提案的目标是进一步开发和调整我们现有的基于DNA的RNAi技术，使其适合高通量基因功能研究和潜在的药物筛选工作的细胞微阵列格式。活细胞阵列的“反向转染法”原理已经建立，并应用于鉴定与细胞生理变化有关的cDNA。我们首先将发表的细胞微阵列方法用于我们的基于DNA的RNAi系统，以证明RNAi微阵列(MAR)的原理。然后我们将使用这种方法来筛选沉默会改变报告基因表达的基因。这种高通量的RNAi形式将对基因分析和药物筛选领域产生巨大的影响。一旦我们开发了这项技术，我们计划利用我们强大的合作和高市场需求，将我们的RNAi系统分布在生物医学领域。

DESCRIPTION (provided by applicant): The goal of this proposal is to further develop and adapt our existing DNA-based RNAi technology to a cell microarray format for high throughput gene function studies and potential drug screening efforts. The principle of arrays of living cells for "reverse transfection" has been established and applied to identifying cDNAs involved in cellular physiology changes. We will first utilize the published cell microarray method for our DNA-based RNAi system to prove the principle of microarrays of RNAi (MAR). We will then use the method to screen for genes the silencing of which will alter the expression of a reporter gene. This high throughput format of RNAi will impact the gene analysis and drug screening fields tremendously. Once we have developed this technology, we plan to take advantage of our strong collaborations and high market demand to distribute our RNAi systems throughout the biomedical field.