Engineering a multi-functional molecular machine for industrial biocatalysis

本项目的目标是开发多功能生物催化剂聚酮合酶（PKS）。该项目将把大型多功能PKS解构成组成部分，研究这些部分如何在隔离状态下工作，以及在体外组合时如何工作，并设计定制的底物特异性，以适应我们的商业重要产品。然后，我们将组装一种有效的生物催化剂，在适当的细菌宿主中合成我们的目标分子。博士课程包括酶工程，测定，蛋白质结构（质谱和X射线晶体学），产品分析，菌株工程/优化的现代方法的培训。您将加入2018-2022年的IBIOIC博士队列，并接受公众参与和业务发展方面的培训。

The goal of this project is to develop polyketide synthase (PKS) enzymes as versatile biocatalysts. The project will deconstruct the large, multi-functional PKSs into component parts, study how the parts work in isolation and when combined in vitro, and engineer bespoke substrate specificity to suit our commercially important products. We will then assemble an efficient biocatalyst that synthesises our target molecules in an appropriate bacterial host. The PhD programme includes training in modern methods of enzyme engineering, assay, protein structure (mass spectrometry and x-ray crystallography), product analysis, strain engineering/optimisation. You will join the IBIOIC PhD cohort for 2018-2022 and also be trained in public engagement and business development.