XRAY DIFFRACTION INVESTIGATION OF CYTOCHROME REDUCTASE

细胞色素还原酶是线粒体的中间部分 呼吸链，在脱氢酶和 细胞色素氧化酶与能量守恒 跨线粒体膜的电化学质子梯度。我们 我收集了重原子到4.5E(完整到约5E)的数据 六方晶体的衍生物，通过与 一种含有汞原子的紧密结合的合成抑制剂。我们 在牛肉的TAMM衍生品中找到了一个重原子位置 水晶。我们已经为牛肉晶体制定了冷冻方案 并从三个新的来源结晶了这种酶，其中两个非常 很有希望。我们需要SR进行更多的筛查和数据收集。

Cytochrome reductase is the middle section of the mitochondrial respiratory chain, passing electrons between the dehydrogenases and cytochrome oxidase and conserving energy in the form of an electrochemical proton gradient across the mitochondrial membrane. We have collected data to 4.5 E (complete to about 5 E) on heavy atom derivatives of the hexagonal crystal produced by co-crystallizing with a tightly-binding synthetic inhibitor containing a mercury atom. We have located a heavy atom site in the TAMM derivative of beef crystals. We have developed a freezing protocol for the beef crystals and crystallized the enzyme from 3 new sources, two of which are very promising. We need SR for more screening and data collection.