ROLE FOR HERPESVIRUS SAIMIRI ORF14 IN TRANSFORMATION & PERSISTENT INFECTION

疱疹病毒开放阅读框14（orft 4）产物 saimiri（HVS）与小鼠乳腺肿瘤具有显著的同源性 病毒超抗原orfl 4编码50-kDa分泌糖蛋白，如 如前所述（Z Yao，E Maraskovsky，M K Spriggs，J I Cohen，R J Armitage和MR Alderson，J Immunol 156：3260-3266，1996）或fl 4 从重组杆状病毒中表达， 来自几种不同来源的CD 4阳性细胞的增殖 为了研究orf 14在转化中的作用， 构建了在orfl 4基因中缺失的HVS（HVS orfl 4）。 在细胞培养物中测试了HVS orf 14的转化潜力， 普通绒猴亲本HVS C亚群488株永生化 体外普通绒猴T淋巴细胞对白细胞介素-2的非依赖性 生长，而HVS orft 4突变体没有产生这样的生长 此外，HVS orf 14通常是非致癌的。 与其他非致癌性HVS突变病毒相反， 从外周血单核细胞中反复分离 感染绒猴超过5个月，HVS或fl 4没有持续存在 这些结果表明，HVS的orf 14是 复制不需要，但转换需要， 对于体内高水平的持久性出版物Duboise，SM，Guo，J， Czajak，S，Lee，H，Veazey，Desrosiers，RC和Jung，JU 松鼠猴疱疹病毒orf 14转化与持续感染J 病毒学1998; 72：6770-6776

The product of open reading frame 14 (orft4) of herpesvirus saimiri (HVS) exhibits significant homology with mouse mammary tumor virus superantigen orfl4 encodes a 50-kDa secreted glycoprotein, as shown previously (Z Yao, E Maraskovsky, M K Spriggs, J I Cohen, R J Armitage, and M R Alderson, J Immunol 156:3260-3266, 1996) orfl4 expressed from recombinant baculovirus powerfully induces proliferation of CD4-positive cells originating from several different species To study the role of orfl4 in transformation, a mutant form of HVS (HVS orfl4) was constructed with a deletion in the orfl4 gene The transforming potential of HVS orfl4 was tested in cell culture and in common marmosets Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, while the HVS orft4 mutant did not produce such a growth transformation In addition, HVS orfl4 was nononcogenic in common marmosets In contrast to oth er nononcogenic HVS mutant viruses which were repeatedly isolated from peripheral blood mononuclear cells of infected marmosets for more than 5 months, HVS orfl4 did not persist at a high level in vivo These results demonstrate that orfl4 of HVS is not required for replication but is required for transformation and for high-level persistence in vivo PUBLICATIONS Duboise, SM, Guo, J, Czajak, S, Lee, H, Veazey, Desrosiers, RC and Jung, JU A role for herpesvirus saimiri orf14 in transformation and persistent infection J Virol 1998; 72:6770-6776