Core--Embryonic stem cell engineering

胚胎干（ES）细胞工程设施的目的是加速依赖于需要ES细胞操作的遗传改变小鼠模型（敲除，敲入和诱导性敲除）的开发的研究计划。成功的ES细胞工程是实现JHU SOM许多NINDS研究人员研究目标的主要障碍（见下文），目前由各个实验室进行，取得了不同程度的成功。ES细胞工程核心将实施ES细胞操作的尖端方法，以确保研究人员使用最有效的策略为NINDS支持的研究生成动物模型。这些策略将包括从现有的小鼠突变系中产生ES细胞，用于随后的基因靶向和嵌合胚胎生产，以及多击 转基因靶向方案，其使用新的重组方法进行受控的定点诱变。通过建立ES细胞工程作为核心资源，我们将标准化最成功的实验室的协议，显着提高模型生成的效率，并加快NINDS支持的研究在JHU SOM的步伐。目前，JHU SOM没有核心设施可以进行ES细胞工程。ES细胞工程核心将与JHU SOM目前的转基因动物设施协同工作， 其提供补充服务，例如将ES细胞注射到胚泡中以及获得高嵌合小鼠所必需的动物饲养。ES细胞工程中心和转基因动物设施提供的服务不会重叠。

The aim of the Embryonic Stem (ES) Cell Engineering facility is to accelerate research programs that depend on the development of genetically altered mouse models (knock-outs, knock-ins, and inducible knock-outs) that require ES cell manipulation. Successful ES cell engineering is a major hurdle for the realization of research goals for many NINDS investigators at JHU SOM (see below), and is currently performed by individual laboratories with varying degrees of success. The ES Cell Engineering Core will implement cutting-edge approaches for ES cell manipulation to assure that investigators use the most efficient strategies to generate animal models for NINDS supported research. These strategies will include generation of ES cells from existing mouse mutant lines for subsequent gene targeting and chimeric embryo production, and multi-hit transgenic targeting protocols that use novel recombination approaches for controlled site-directed mutagenesis. By establishing ES cell engineering as a core resource we will standardize protocols from the most successful laboratories, markedly increase the efficiency of model generation, and speed the pace of NINDS-supported research at JHU SOM. There is currently no core facility at JHU SOM that can perform ES cell engineering. The ES Cell Engineering Core will work in synergy with the current Transgenic Animal facility at JHU SOM, which provides complementary services such as injection of ES cells into blastocysts and animal husbandry necessary to achieve high chimera mice. There will be no overlap in the services provided by the ES Cell Engineering Core and the Transgenic Animal facility.