CORE--SPECTROSCOPY SUPPORT

光谱支持设施(SSF)提供的服务涵盖两个不同的领域： 1)核磁共振、圆二色谱和荧光的应用 用于研究生物分子结构和功能的光谱学，以及2)磁共振 用于小动物体内成像的显微镜(MRM)，特别是与癌症研究相关的。该设施拥有两台设备齐全的核磁共振仪器，一个带有Bruker DMX-600控制台的600 MHz(14特斯拉)磁体，一个带有Bruker DRX-300控制台的大口径300 MHz(7特斯拉)磁体，包括一个Biospec显微成像附件。设施人员维护和操作光谱仪，执行核磁共振分析和小动物成像服务，并为用户提供专家建议和技术支持。这台600 MHz的仪器配备齐全，可用于研究生物大分子的结构、动力学和相互作用的异核多维核磁共振。300 MHz仪器非常适合小分子的多核分析和涉及同位素标记配体的酶功能研究。对于研究老鼠癌症模型的研究人员来说，300 MHz仪器上的小动物成像是有兴趣的。MRM可以监测肿瘤的发展、生长、灌注、血管形成和治疗反应。为了在小动物模型中研究表达发光或荧光蛋白的肿瘤，正在实施新的光学成像能力。在最后一次审查中，光谱支持设施被评为“非常好到极好”。2003年，来自所有三个部门的9个不同项目的18名经同行审查、资助的调查人员使用了该机制。这比五年前的用户数量增加了80%，解决了之前的担忧，即该设施主要为生物分子结构和功能计划中的少数研究人员提供服务。该设施的广泛影响在很大程度上是由于实施了小动物成像，这使得来自所有三个部门的另外八个用户能够支持与癌症相关的研究项目。在MR ilmaging服务完全投入使用后，300 MHz仪器的使用量增加了两倍。超过99%的总使用量是由同行审查、资助的调查人员提供的。在该机制的帮助下获得的数据有 在过去五年中，在同行评议的期刊上发表了27篇文章。

The services offered by the Spectroscopy Support Facility (SSF) cover two distinct areas: 1) applications of nuclear magnetic resonance (NMR), circular dichroism, and fluorescence spectroscopy for studies of biomolecular structure and function, and 2) magnetic resonance microscopy (MRM) for In vivo imaging of small animals especially relevant to cancer research. The Facility houses two well equipped NMR instruments, a 600 MHz (14 Tesla) magnet with a Bruker DMX-600 console, a wide-bore 300 MHz (7 Tesla) magnet with a Bruker DRX-300 console, including a Biospec microimaging accessory. Facility personnel maintain and operate the spectrometers, perform NMR analytical and small-animal imaging services, and offer expert advice and technical support to users. The 600 MHz instrument is fully equipped for heteronuclear multidimensional NMR studies on the structure, dynamics and interactions of biological macromolecules. The 300 MHz instrument is well suited for multinuclear analysis of small molecules and studies of enzyme function involving isotopically-labeled ligands. Small-animal imaging on the 300 MHz instrument is of interest to investigators studying mouse models of cancer. MRM permits monitoring of tumor development, growth, perfusion, vascularization, and response to treatment. New capabilities for optical imaging are being implemented in order to study tumors expressing luminescent or fluorescent proteins in small-animal models. The Spectroscopy Support Facility was rated "Very Good to Excellent" at the last review. In 2003, the Facility was used by 18 peer-reviewed, funded investigators from 9 different Programs in all three Divisions. This represents an 80% increase over the number of users five years ago and addresses the previous concern that the Facility primarily served a small number of investigators in the Biomolecular Structure and Function Program. The broadened impact of the Facility is largely due to the implementation of small animal imaging, which made it possible to support cancer-related research Programs by eight additional users from all three Divisions. After the MR ilmaging services became fully operational, the usage of the 300 MHz instrument increased by two-fold. Over 99% of the total usage is by peer-reviewed, funded investigators. Data obtained with the help of the Facility have resulted in 27 publications in peer-reviewed journals over the past five years.