CORE--DNA SEQUENCING FACILITY

自动DNA测序设备是一个合并的设备，为研究人员提供 最新的DNA测序和DNA多态分析。该设施有52人使用 来自所有三个部门的10个研究项目的同行评议资助的调查人员。99.5%(99.5%)的用户获得了同行评审的资金。理查德·哈迪博士(免疫生物学项目负责人)于1993年担任该机构的负责人。使用ABI Model 373的自动测序是在1994年建立的。随后又增加了377型测序仪，一个在1995年，另一个在1997年。第三架377在2002年取代了最初的373。最近，两台377已经被现代的ABI 3100毛细管仪器取代，提供了更可靠的高通量测序。测序设备为Fox Chase癌症中心(Fccc)的研究人员提供了计算机可读的DNA样本序列，与手动测序相比，生成数据更快，读取长度更长。研究人员使用自动序列分析来验证DNA结构，识别突变，并确定新克隆基因的结构。使用自动序列分析对大基因(5-20kb)进行测序要容易得多，与使用手动测序的几个月相比，在几周内就能产生结果。该设施的使用量在过去五年中继续稳步增长，从1998年的17,000个序列增加到2003年的28,000个序列(增长了%)。除了测序外，在基因图谱研究中很重要的DNA多态分析也可以在377上使用“GeneScan”软件在设施中使用。公平地获得 该设施由一个院系监督委员会保证。该设施被评为“杰出的” CCSG的最后一次审查。

The Automated DNA Sequencing Facility is a merged Facility which provides investigators with state-of-the-art DNA sequencing and analysis of DNA polymorphisms. The Facility is used by 52 peer-reviewed funded investigators across 10 Research Programs from all three Divisions. Ninety-nine and one-half percent (99.5%) of the users have peer-reviewed funding. Richard Hardy, Ph.D. (Program Leader, Immunobiology) assumed direction of the Facility in 1993. Automated sequencing using an ABI Model 373 was set up in 1994. Model 377 sequencers were subsequently added, one in 1995, and another in 1997. A third 377 replaced the original 373 in 2002. Recently, two of the 377s have been replaced by a modern ABI 3100 capillary instrument, providing more reliable high-throughput sequencing. The Sequencing Facility provides Fox Chase Cancer Center (FCCC) investigators with computer-readable sequences of their DNA samples in a timely and cost effective manner, generating data more rapidly and of greater read-length compared to manual sequencing. Automated sequence analysis is used by investigators to verify DNA constructs, to identify mutations, and to determine the structure of newly cloned genes. Sequencing large genes (5-20 Kb) is much more tractable using automated sequence analysis, generating results in a few weeks, as compared to months using manual sequencing. The volume of usage of the Facility has continued to increase steadily over the past five years from 17,000 sequences in 1998 to over 28,000 in 2003 (64% increase). In addition to sequencing, analysis of DNA polymorphisms, important in genetic mapping studies, is also available in the Facility using "GeneScan" software on the 377. Equitable access to the Facility is assured by a Faculty Oversight Committee. The Facility was rated "Outstanding" at the last CCSG review.