Purchase of Mass Spectrometric Instrumentation

描述(由申请人提供)：为加州大学洛杉矶分校帕萨罗质谱学实验室购买配备电喷雾、纳米喷雾和常压化学电离源的高性能LC/MS/MS系统所需资金。这是一个由化学和生物化学系(文理学院)、精神病学和生物行为科学系以及神经精神病学研究所(医学院)共同支持的共享设施，可供整个加州大学洛杉矶分校的校园研究和教学企业使用。这一应用背后的驱动力是需要一种仪器来专门分析脑透析液组织灌注活检样本，以便对同一样本中的多种神经递质和相关化合物进行特定的定量，并识别与已知神经递质共同释放的新型神经调节化合物。加州大学洛杉矶分校至少有七个不同的实验室将在他们的研究中使用这种仪器。在这项研究中，对灵敏度的极端要求需要一种可以保留下来以绝对最高效率运行的仪器。加州大学洛杉矶分校的校园里没有适合这类研究和这类样本的仪器。这一仪器将成为加州大学洛杉矶分校越来越多的科学家使用脑透析作为核心实验技术的核心分析工具，获得这一仪器对这些项目的持续生产力、成功和竞争力至关重要。PI和他的同事仔细评估了所有可能满足苛刻要求的可用仪器，并选择了应用生物系统公司的Q Trap Pro LC/MS/MS系统包作为目前可用的最合适的仪器。介绍了该仪器的七个主要用户的研究情况。

DESCRIPTION (provided by applicant): Funds are requested to purchase a high performance LC/MS/MS system equipped with electrospray, nanospray and atmospheric pressure chemical ionization sources for the UCLA Pasarow Mass Spectrometry Laboratory. This is a shared facility jointly supported by the Department of Chemistry & Biochemistry (College of Letters and Sciences) and the Department of Psychiatry & Biobehavioral Sciences and the Neuropsychiatric Institute (School of Medicine) that is available to the entire UCLA campus research and teaching enterprise. The driving force behind this application is the need for an instrument that can be devoted to analyses of brain dialysate tissue perfusion biopsy samples for the specific quantification of multiple neurotransmitters and related compounds in the same samples, and for the identification of novel neuro-regulatory compounds, which are co-released with known neurotransmitters. At least seven different laboratories on the UCLA campus will use this instrument in their research. The extreme demands for sensitivity in this research require an instrument that can be reserved for operation at absolute maximal efficiency. There is no instrument on the UCLA campus, which is available and suitable for this type of research and these types of samples. This instrument will be a central analytical tool of the growing cadre of UCLA scientists who use brain dialysis as a core experimental technique, and the acquisition of this instrument is essential for the continued productivity, success and competitiveness of these programs. The PI and his associates have carefully evaluated all available instruments which potentially fulfill the demanding requirements, and have chosen the Q Trap Pro LC/MS/MS system package from Applied Biosystems as the most suitable of those currently available. Descriptions of the research of the seven major users of the instrument are provided.