Optical Imaging of S1 Cortex in the Awake Primate

描述(由申请人提供)：这项研究提案的目标是开发用于清醒的、行为正常的猴子的光学成像方法。光学成像已经非常成功地揭示了麻醉动物的皮质组织。我们建议发展这种方法来研究麻醉清醒猕猴躯体感觉皮层躯体感觉生理反应中的皮质激活模式。然而，人们对清醒的行为动物的激活空间模式知之甚少。所提出的方法是研究躯体感觉皮质功能的一种非常新颖的方法。通过将大脑皮质激活模式的高空间和时间分辨率图像与行为直接联系起来，它有望对大脑皮质功能的观点产生高度影响。我们有两个目标。其中一个目标是开发一种能够同时具有高空间和时间分辨率的光学成像系统，用于清醒、行为正常的猴子。本征信号和电压敏感染料成像功能将整合到一个单一系统中。这包括硬件和软件的开发，在猴子身上进行测试，以及开发新的数据分析方法。我们的第二个目标是对执行触觉任务的清醒猴子进行探索性光学成像研究。麻醉和清醒的SI在地形、功能组织和区域间激活方面有显著差异。我们的目标是探索这些差异是否与注意力和任务依赖背景等因素相关。鉴于我们的初步数据，这些研究极有可能被证明是卓有成效的，并将成为未来R01表现猴子的基础。提出的方法论是新颖的，将为认知功能的研究提供一种新的途径。此外，在猴子身上进行的这种类型的功能成像将在单一单位生理学、功能磁共振成像和行为研究的身体之间提供亟需的联系。

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop the optical imaging methodology for use in awake, behaving monkeys. Optical imaging has been highly successful in revealing cortical organization in anesthetized animals. We propose to develop this method to study cortical activation patterns in somatosensory physiological responses of somatosensory cortex in anesthetized an awake monkeys. However, little is known about the spatial patterns of activation in awake, behaving animals. The proposed methodology is a highly novel approach to the study of somatosensory cortical function. By directly relating high spatial and temporal resolution images of cortical activation patterns to behavior, it promises to have a high impact on views of cortical function. We have two aims. One aim is to develop an optical imaging system capable of both high spatial and temporal resolution for use in awake, behaving monkeys. Both intrinsic signal and voltage sensitive dye imaging capabilities will be incorporated into a single system. This involves hardware and software development, testing in behaving monkeys, and development of new data analysis methods. Our second aim is to do exploratory optical imaging studies in awake monkeys performing tactile tasks. There are significant differences in topography, function organization, and inter-areal activations between anesthetized an awake SI. We aim to explore whether these differences correlate with factors such as attention and task- dependent context. Given our preliminary data, it is highly likely that these studies will prove fruitful and will form the basis for future R01s in behaving monkeys. The proposed methodology is novel and will permit a new approach to the study of cognitive functions. Further, this type of functional imaging in behaving monkeys will provide a much needed link between bodies of single-unit physiology, fMRI, and behavioral studies.