AGING, STRESS, OBESITY AND CHRONIC INFLAMMATION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。65岁以上的人是我们人口中增长最快的部分。关节炎、癌症、糖尿病、心血管疾病和痴呆症是构成这些老龄化疾病的最大组成部分。在这些疾病的发病机制中似乎起主要作用的两个因素是心理社会因素和慢性炎症。压力、社会经济地位、抑郁、孤独、悲观和许多不良的健康行为是与这些疾病的病因和促进有关的几个心理社会因素。慢性炎症在这些疾病中的重要性从关节炎和心血管文献中的大量报告中获得了可信度。另一个与慢性炎症相关的国家健康问题是肥胖，它是所有老年病的风险因素。在这项提案中，我们将评估IL-6和另一组生物、心理、社会和基因组因素，这些因素已被证明或假设与炎症级联有关。我们将探讨低SES应激与慢性炎症之间的关系。此外，我们还将评估可能改变或混淆这一效应的其他生物、心理和社会因素。这项初步研究将包括评估一组(n=60)35-60岁超重男性或女性的社会经济地位低(每年15-30,000美元)和高(每年50-80,000美元)，评估与慢性炎症有关的各种基因组和生物心理社会因素。将分别使用静脉穿刺法和唾液技术获取血清和唾液样本。在最初的研究访问后的4周内，将评估两组受试者的恢复力(从日常应激源中恢复的能力)与炎症反应的关系，这是通过每日日记方法确定的。每周对皮质醇分泌的评估将为这项评估增加一项生物应激措施。C反应蛋白和IL-6将在每月日记评估的第二周末和月末抽查。在月底之前和月底，我们将获得与慢性炎症相关的额外生物标记物，并将它们与低SES受试者在本月的压力和韧性相关联。对于高SES组，患者将被要求返回进行相同的评估，但我们不会要求他们使用日记。我们对低SES组的弹性变化特别感兴趣，来自这些患者的日记数据将有助于设计针对他们的干预措施。将在基线时进行单层腹部CT扫描，以提供内脏脂肪含量的信息。一部分人还将接受核磁共振检查，以获得有关所有身体脂肪沉积的信息。预计这项研究将产生初步数据，并支持R-21和项目项目向国家老龄研究所提交关于压力、衰老和慢性炎症的报告。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. People over 65 represent the fastest growing segment in our population. Arthritis, cancer, diabetes, cardiovascular disease, and dementia are diseases that make up the largest component of these diseases of aging. Two elements that appear to play a major role in the pathogenesis of these disorders are psychosocial factors and chronic inflammation. Stress, socioeconomic status, depression, loneliness, pessimism, and numerous poor health behaviors are a few of the psychosocial factors that have been implicated in the etiology and promotion of these disorders. The importance of chronic inflammation in these disorders gains credibility from numerous reports in the arthritis and cardiovascular literature. Another national health problem associated with chronic inflammation is obesity which is a risk factor for all of the diseases of aging.. In this proposal we will be evaluating IL-6 and an additional set of biopsychosocial and genomic factors that have been demonstrated or postulated to be linked to the inflammatory cascade. We will explore the association between the stress of low SES and chronic inflammation. Additionally, we will evaluate other biopsychosocial factors that may modify or confound this effect. This pilot study will consist of evaluating a cohort (n=60) of 35-60 year old overweight males or females of low ($15-30,000 per annum) and high ($50-80,000 per annum) socioeconomic status, over 1 month, assessing various genomic and biopsychosocial factors related to chronic inflammation. Venipuncture and salivette techniques will be used to obtain serum and saliva samples, respectively. During the 4 weeks after the initial study visit, the relationship of resiliency (ability to recover from daily stressors), as determined by daily diary methods, to the inflammatory response will be evaluated on the subjects in the both grouped. Weekly assessments of cortisol secretion will add a biologic stress measure to this evaluation. CRP and IL-6 will be drawn at the end of the second week of the monthly diary assessment and at the end of the month. Before and at the end of the month we will acquire an additional battery of biomarkers associated with chronic inflammation and correlate them with the low SES subjects' stress and resilience during the month. For the high SES group, patients will be asked to return for the same assessment, but we will not ask them to use the diaries. We are particularly interested in the variation in resiliency in the low SES group, and the diary data from these patients will be helpful in designing an intervention specific to them. A single cut abdominal CT scan will be obtained at baseline to provide information on visceral fat content. A subset of individuals will also receive an MRI to obtain information on all body fat deposits. It is anticipated that this study will yield preliminary data and support for an R-21 and Program Project submission on Stress, Aging and Chronic Inflammation to the National Institute of Aging.