Biophotonic detection of WNV Abs from Multiple Species

描述（由申请人提供）：本2期提案将进一步开发一种全新的、广泛适用的技术（鸭嘴兽技术），用于快速、无标记检测蛋白质。在第1阶段，我们展示了使用液晶结合亲和接触印刷检测来自多个物种和多个同种型的抗体。在这项提案中，我们开发了替代显微镜和相机依赖的方法来分析这项技术，我们将该技术转化为多孔格式，适用于快速，定制的多路复用和适合半自动化高通量测试。我们还证明了抗体检测方法的通用性，我们将鸭嘴兽抗体测定与ELISA和SN检测抗体进行了比较，使用来自WNV感染的马和禽类的血清。

DESCRIPTION (provided by applicant): This Phase 2 proposal will further the development of a fundamentally new, broadly applicable technology (Platypus technology) for the rapid, label-free detection of proteins. In Phase 1, we demonstrated the use of liquid crystals in combination with affinity contact printing for the detection of antibodies from multiple species and of multiple isotypes. In this proposal, we develop alternatives to microscopic and camera dependent methods for analysis of this technology and we transform the technology into a multi-well format, suitable for rapid, customized multiplexing and appropriate for semi-automated high throughput testing. We also demonstrate the generality of the approach for antibody detection and we com pare Platypus Antibody Assay with ELISA and SN detection of antibodies using sera from WNV infected horses and avians.