NONINVASIVE DETECTION OF NEW SIV LINEAGES IN CAPTIVE SOOTY MANGABEYS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们建立了一种非侵入性的方法检测SIVsm病毒粒子RNA在粪便中的圈养mangabeys（SMs）（Cercocebusatys）。利用这种方法研究野生濒危SM中SIVsm的自然历史，有助于了解SIVsm和HIV-2的多样性和进化。粪便样品收集61野生SM和14野生西非黑猩猩（黑猩猩verus）进行了研究。1993年在塞拉利昂农村地区采集了样本。1份SM样品经逆转录酶-PCR检测呈阳性。在14只黑猩猩粪便中未检测到病毒序列。对从SM#13获得的env序列的系统发育分析显示，它在包括SIVsmH 4、B670和PBj的SIVsm组内聚类，鉴定了来自西非和美洲SM菌落的先前未知的SIV连接。该病毒命名为SIVsmSL 93 g，表明SIVB 670-SIVsmH 4-SIVPbj簇起源于塞拉利昂北方。在野生SM中发现该毒株也表明，非侵入性方法可用于从野外收集的猴粪便中检测SIV。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We developed a noninvasive method for SIVsm virion RNA detection in feces of captive sooty mangabeys (SMs) (Cercocebus atys). Employing this method to investigate the natural history of SIVsm in endangered SMs in the wild is useful for understanding diversity and evolution of SIVsm and HIV-2. Fecal samples collected from 61 wild SMs and 14 wild west African chimpanzees (Pan troglodytes verus) were studied. Samples were collected in rural Sierra Leone in 1993. One SM sample tested positive by reverse transcriptase-PCR. No viral sequences were detected in 14 feces of chimpanzees. Phylogenetic analysis of env sequence obtained from SM #13 showed that it clustered within the SIVsm group that includes SIVsmH4, B670 and PBj, identifying a previously unknown SIV connection from West Africa and American based colonies of SM. The virus, designated as SIVsmSL93g, indicates that the SIVB670-SIVsmH4-SIVPbj cluster originated in Northern Sierra Leone. The discovery of this strain in wild SM also indicates that noninvasive methods can be used for SIV detection from monkey feces collected in the field.