BECTON DICKENSON FACSARIA CELL SORTER: IMMUNOLOGY, MALARIA

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：我们正在申请资金购买Becton Dickenson FACSAria高速细胞分选机。所要求的配置包括三个激光器，能够检测多达九种不同的荧光染料，具有一系列的激发和发射特性。该仪器还包括收集和控制单个分类细胞的能力。新仪器将用于一些分析和分类应用程序，以支持国家卫生研究院资助的项目。这些应用包括细胞信号分子的多重免疫荧光分析，从动物组织中分离白细胞，以及筛选高亲和力抗体和多肽的酵母展示文库。新仪器将被安置在LJBI流式细胞仪设施中。已经制定了准入和使用政策，并制定了机构承诺，为最初三年的运作提供人员和维护方面的支持。FACSAria在LJBI先进的流式细胞仪开发和应用能力的开发中发挥了关键作用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a Becton Dickenson FACSAria high speed cell sorter. The requested configuration includes three lasers, enabling the detection as many nine different fluorochromes with a range of excitation and emission properties. The instrument also includes capabilities for the collection and temperature control of single sorted cells. The new instrument will be used for a number of analysis and sorting applications in support of NIH-funded projects. These applications include multiplexed immunofluorescence analysis of cell signaling molecules, sorting of leukocytes from animal tissue, and the screening of yeast display libraries for high affinity antibodies and polypeptides. The new instrument will be housed in the LJBI Flow Cytometry Facility. Policies for access and use have been formulated, and an institutional commitment is in place to support personnel and maintenance for the initial three years of operation. The FACSAria plays a key role in the development of an advanced flow cytometry development and application capability at LJBI.