Time-Of-Flight Instrument with Variable Ionization Sources

描述（由申请人提供）：具有可变电离源的飞行时间仪器：我们在宾夕法尼亚大学化学系的质谱设备中发现了两个非常关键的问题：(1)缺乏常规测量大范围化合物的高分辨率数据的能力；(2)缺乏常规分析较大合成分子和生物分子的能力。安捷伦公司所要求的飞行时间（TOF）仪器将彻底解决这些关键问题。该仪器由TOF质谱分析仪和多个大气压电离源组成。TOF分析仪旨在同时提供卓越的质量精度（< 3ppm），高质量分辨率（> 13,000），大动态范围（3.5十年）和快速的光谱采集速率（20-40光谱/秒），质量/电荷（m/z）范围为50-12,000。多种大气压电离源包括电喷雾电离（ESI）、大气压化学电离（APCI）和基质辅助激光解吸/电离（MALDI）。选择该仪器是因为它独特地结合了(1)在大m/z范围内确定精确质量的能力，(2)由于可互换的电离源，分析非常广泛的化合物类别的能力，以及(3)因为仪器和软件的设计允许以常规的步行方式测量样品。这三个特性使该仪器非常适合宾夕法尼亚大学化学系的多用户环境。该仪器将满足12名nih支持的研究人员（主要用户群体）对合成和天然分子（如小分子、小分子和天然产物、非天然低聚物、天然和修饰肽、拟肽、天然和人工寡核苷酸和小蛋白质）的设计、合成和评估的所有关键和当前未满足的需求。据估计，在五年内，该仪器将显著影响大约20名美国国立卫生研究院资助的宾夕法尼亚大学化学系研究人员的研究项目。

DESCRIPTION (provided by applicant): Time-Of-Flight Instrument with Variable Ionization Sources: We identified two very critical problems in the mass spectrometry facility of the Department of Chemistry at the University of Pennsylvania: (1) A lacking capability to routinely measure high-resolution data for a broad range of compounds and (2) a lacking capability to routinely analyze larger synthetic molecules and biomolecules. The requested Time-Of-Flight (TOF) instrument from Agilent will completely solve these critical problems. The proposed instrument consists of a TOF mass analyzer and multiple atmospheric pressure ionization sources. The TOF analyzer is designed to simultaneously provide excellent mass accuracy (< 3 ppm), high mass resolution (> 13,000), large dynamic range (3.5 decades), and fast spectral acquisition rates (20-40 spectra/sec) for the mass/charge (m/z) range of 50-12,000. The multiple atmospheric pressure ionization sources include electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and matrix- assisted laser desorption/ionization (MALDI). The instrument was selected because it uniquely combines (1) the ability to determine accurate masses over a large m/z range, (2) the capability to analyze a very broad range of compound classes due to interchangeable ionization sources, and (3) because the design of the instrument and the software allow the measurement of samples in a routine walk-up fashion. These three properties make this instrument ideal for the multi-user environment in the Chemistry Department at the University of Pennsylvania. The proposed instrument will meet all the critical and currently unfulfilled needs of 12 NIH-supported researchers (major user group) concerned with the design, synthesis, and evaluation of synthetic and natural molecules such as small molecules, small and natural products, unnatural oligomers, natural and modified peptides, peptidomimetics, natural and artificial oligonucleotides, and small proteins. It is estimated that over five years, the instrument would impact significantly the research programs of around 20 NIH-funded investigators in the Chemistry Department at the University of Pennsylvania.