Infrastructure for Human Embryonic Stem Cells Productio*

这项提议的主要目的是促进用于人类胚胎干细胞的基本方法学。 (HESCs)培养，以鼓励它们在无饲养层、无动物和无血清的条件下大规模生长 条件及其分布。此外，我们的目标是进一步鉴定这些细胞并培养它们。 根据良好实验室操作规范(GLP)的规定。通过实现这些目标，我们将能够分发HESCs 这不仅适用于基础研究，而且有可能用于临床和工业用途。 为了实现这些目标，我们的研究团队将专注于以下几个方面； 探索hESCs的最佳培养条件：饲养层、动物和无血清条件。 在GLP中培养和繁殖hESCs。 ？生成一组用于评估电池质量的测量。 4种美国国立卫生研究院注册细胞在不同环境中培养过程中的综合特征 培养条件、长时间培养后和早期分化过程中。 改进现有的冷冻和解冻胚胎干细胞的方法。 这些改进将构成在以下条件下大量培养和繁殖人类胚胎干细胞的能力 促进这些细胞的研究和临床应用。

The main aim of this proposal is to promote the basic methodology used for human embryonic stem cells (hESCs) culturing in order to encourage their large-scale growth in feeder layer-, animal- and serum-free conditions, and their distributions. In addition, we aim to further characterize these cells and to culture them under good laboratory practices (GLP) regulations. By achieving these goals, we will be able to distribute hESCs that will not only be suitable for basic studies but also potentially for clinical and industrial uses. To meet these aims our research team will concentrate on the following; ¿ Exploring the optimal culture conditions for hESCs: feeder layer-, animal- and serum-free conditions. ¿ Growing andpropagating hESCs inGLP. ¿ Generating a setof measurements for the evaluation ofthe cells' quality. ¿ Characterizing comprehensively the four Technion NIH-registered cells during their culture in various culture conditions, after prolonged culture and during early differentiation events. ¿ Improving the existing methods forfreezingandthawinghESCs. These improvements will constitute the ability to grow and propagate hESCs in large quantities in conditions that promote both research and clinical utilization of these cells.