Zeiss/31 Stallion DDI Multi-Dimensional Live-Cell Imaging Workstation

描述(由申请人提供)：约翰霍普金斯大学文理学院、工程学院、医学学院和公共卫生学院的11名由NIH资助的研究人员；以及马里兰大学医学院的马里兰大学生物技术研究所申请资金，从Carl Zeiss/智能成像创新公司购买Stallion DPI Live-Cell数字成像工作站。所要求的系统集成了蔡司200M TIRF平台和两个超高速/高灵敏度Cascade 512B EM数码相机；一个405/488 FRAP/光激活激光模块；完全集成的31个幻灯片本多维采集/分析软件；并且针对1)快速/超灵敏4D双通道采集；2)反褶积；3)FRFT和比率成像；4)粒子跟踪；以及5)FRAP和光激活进行了优化。新的最先进的工作站将补充我们经常使用的蔡司LSM 510 META和老化的Deltavision系统，同时在速度和灵敏度方面进行重大升级。重要的是，种马DPI将为霍普金斯综合成像中心提供新的/独特的宽视场荧光能力，这是霍姆伍德校园/霍普金斯大学范围内的显微资源，由60个实验室和约200名用户组成的多个学校和部门定期使用。我们的研究人员都是由NIH资助的，他们致力于一系列基本的细胞生物学、生化和生物分子方面的问题，包括但不限于：肿瘤中的端粒/着丝粒重组；HSV组装和成熟；MHC I运输和组装、膜组织和动力学；内小体/溶酶体动力学；膜运输；聚合基因载体的运输；线粒体和细胞凋亡；唾液酸显示和细胞黏附；以及内吞作用的机制。在要求的配置中，Stallion DDI工作站将为我们不同的研究人员小组提供广泛的基本/高级活细胞显微镜功能，这些功能目前在霍普金斯大学霍姆伍德园区是不足的/缺乏的。新系统将在生物系的综合成像中心(否：否)方便地安装、管理和维护，它将并入IIC现有的、完善的充值系统，以确保收回用品和维护资金。

DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.