Mouse Models Core

小鼠模型核心设施的主要目的是熟练负担得起的一代遗传 Moffitt研究人员为基础和转化癌症研究改造小鼠。进展 通过基因表达、突变或缺失策略对实验室小鼠进行实验操作 已经推动了发育生物学、生理学和病理学领域的重大进展。转基因 和靶向小鼠在体内环境中充当基因功能（或功能障碍）的无与伦比的模型， 从而大大有助于我们理解动态的、复杂的致癌机制。这些模型 在癌症和其他疾病的预防、治疗和病因学研究中也是非常宝贵的。为 研究人员认为，研究这些小鼠模型的缺点或阻碍是大量的成本，时间， 设备和专业知识所需的推导和检查。鼠标模型核心还提供 荧光素酶表达肿瘤细胞系和Xenogen MS工作站的生物发光成像， 改进了传统异种移植小鼠模型的研究。 该核心的具体目标是： - 就转基因和靶向载体的总体策略和设计向研究者提供建议; - 提供相关质粒载体和λ基因组文库; - 提供胚胎干细胞及其培养、转染、选择、筛选指导; - 提供原核DMA显微注射服务，生成转基因创始小鼠; - 提供囊胚显微注射服务，从靶向ES细胞生成生殖系嵌合体; - 在小鼠的基因型和表型分析中提供建议和协助; - 提供基因工程小鼠模型的冷冻保存和无病原体的再衍生;以及 - 为异种移植小鼠模型提供标记有端粒酶的肿瘤细胞系和生物发光成像。

The primary purpose of the Mouse Models Core Facility is the proficient affordable generation of genetically modified mice for both basic and translational cancer research by Moffitt investigators. Advances in experimental manipulation of the laboratory mouse via gene expression, mutation, or deletion strategies have propelled major progress in the fields of developmental biology, physiology, and pathology. Transgenic and targeted mice serve as incomparable models of gene function (or dysfunction) in context in vivo and thereby contribute greatly to our understanding of dynamic, complex oncogenic mechanisms. These models are also invaluable in studies of the prevention, treatment, and etiology of cancer and other diseases. For investigators, the disadvantages or deterrents to studying these mouse models are the substantial cost, time, equipment, and expertise required for their derivation and examination. The Mouse Models Core also offers luciferase expressing tumor cell lines and bioluminescent imaging with the Xenogen MS workstation for improved study of traditional xenograft mouse models. The specific objectives of the core are to: - Advise investigators on the overall strategy and design of transgenic and targeting vectors; - Provide relevant plasmid vectors and lambda genomic libraries; - Provide embryonic stem (ES) cells and instruction in their culture, transfection, selection, screening; - Provide pronuclear DMA microinjection services to generate transgenic founder mice; - Provide blastocyst microinjection services to generate germline chimeras from targeted ES cells; - Advise and assist investigators in the genotypic and phenotypic analyses of mice; - Provide cryopreservation and pathogen-free rederivation of genetically engineered mouse models; and - Provide luciferase-tagged tumor cell lines and bioluminescent imaging for xenograft mouse models.