GNRH II STIMULATION AND SEXUAL DYSFUNCTION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。为了加深对绒猴神经内分泌机制的了解，阐明GnRH多肽作为非雌激素刺激因子对女性反应的有效性，并帮助开发更合适的临床干预措施来治疗女性性功能障碍。据估计，43%的女性会受到性功能障碍的影响。目前还没有批准的药物疗法来治疗这种疾病。进一步考虑到雌激素和孕激素的激素治疗并不安全，促使人们寻找非类固醇药物治疗。神经肽促性腺激素释放激素II刺激一系列非灵长类动物的性行为，但它在人类和非人类灵长类动物中的作用尚不清楚。我们开发了一个以雌性普通绒猴为对象的模型系统来确定对GnRH II的行为反应。与对照组相比，GnRH II使雌性对伴侣表现出的促进性(性引诱)行为(挥动舌头、诱导性凝视和僵硬姿势)的频率增加了近四倍。GnRH II通过在不影响坐骑或坐骑尝试频率的情况下，将雄性的插入频率增加一倍，从而提高了性接受能力(雌性使男性发生性行为)。促性腺激素释放激素II对性行为的刺激不依赖于雌二醇。这些发现表明GnRH II在调节雌性灵长类动物的性行为中发挥了作用。供资在本报告所述期间之前结束，本进度报告是关于由此产生的调查结果和出版物。这项研究使用了WNPRC化验服务和动物服务。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To improve our understanding of neuroendocrine mechanisms in marmosets to clarify the efficacy of GnRH peptides as non-estrogenic stimulators of female response and aid in developing more appropriate clinical intervention for sexual dysfunction in women. Sexual dysfunction affects an estimated 43% of women. Currently there are no approved drug therapies for this disorder. Further concerns that hormonal treatment with estrogens and progestogens are not safe motivates the search for non-steroidal pharmoacotherapy. Neuropeptide gonadotropin releasing hormone II stimulates sexual behavior in a range of non-primates, but its role in humans and nonhuman primates is unknown. We developed a model system with female common marmosets to determine the behavioral responses to GnRH II. GnRH II increases nearly four-fold the frequency of proceptive (sexual solicitation) behaviors (tongue flicking, proceptive stares and frozen postures) exhibited by females towards their pair-mates compared to controls. GnRH II increases sexual receptivity (females enabling male sexual behavior) by doubling the frequency of intromission by males compared to vehicle without affecting mount or mount attempt frequencies. GnRH II stimulation of sexual behavior is not estradiol dependent. These findings indicate a role for GnRH II in the regulation of female primate sexual behavior. The funding ended before this reporting period and this progress report is on the resulting findings and publication. This research used WNPRC Assay Services and Animal Services.