ENGINEERED GLUCOSE METABOLISM IN INSULIN SECRETING CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。纽加德实验室采用跨学科的方法来研究葡萄糖刺激胰岛素分泌的生化和分子机制，包括开发新的细胞模型，基因发现和基因工程。该项目目前的研究重点是丙酮酸循环改变（通过13C核磁共振测量）在葡萄糖刺激胰岛素分泌（GSIS）损伤中的潜在作用，如在糖尿病中发现。《资源》先前的研究使用13C核磁共振表明，在高水平的游离脂肪酸（1mm油酸：棕榈酸混合物）中长期（48小时）培养的葡萄糖反应强烈的细胞系显示出丙酮酸循环减少和GSIS。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Newgard laboratory uses interdisciplinary approaches for the study of the biochemical and molecular mechanisms involved in glucose-stimulated insulin secretion, including development of novel cellular models, gene discovery and genetic engineering. Current studies in this project focus on the potential role of altered pyruvate cycling (as measured by 13C NMR) in impairment of glucose-stimulated insulin secretion (GSIS) such as found in diabetes. Previous studies in the Resource used 13C NMR to show that robustly glucose responsive cell lines subjected to chronic (48 h) culture in elevated free fatty acids (1 mM oleate:palmitate mixture) displayed decreased pyruvate cycling and GSIS.