HEPATITIS C VIRUS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。丙型肝炎病毒是一种血液传播的病原体，感染了大约3%的人口。超过70%的感染该病毒的人最终会患上慢性肝炎，这可能会导致肝硬变、肝细胞癌、自身免疫性疾病等近30种其他疾病。最初，丙型肝炎病毒感染是无症状的。感染20-30年后，大多数患者会出现慢性肝脏感染的症状(乏力和食欲不振)，并伴有AST和ALT等肝酶水平升高1)利用在肝损伤或特异性抗丙型肝炎抗体形成之前感染早期收集的感染血液，通过冷冻EM确定丙型肝炎病毒(HCV)的3D结构及其分子组成2)分析包膜蛋白E1E2的分子结构，并建立与细胞受体相互作用的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HCV is a blood born pathogen that infects about 3% of the human population. More than 70% of people infected with the virus eventually get chronic hepatitis which can cause cirrhosis of the liver, hepatocellular carcinoma, autoimmune diseases, and almost 30 other associations. Initially, HCV infection is asymptomatic. After 20-30 years of infection, most of patients will have symptoms (fatigue and poor appetite) of chronic liver infection with elevated levels of liver enzymes such as AST and ALT 1) Determine the 3D structure of hepatitis C virus (HCV) and its molecular components by cryo-EM using infectious blood collected during the early stages of infection prior to the development of liver injury or specific anti-HCV antibody 2) Analyze the molecular structure of the envelope proteins, E1E2, and develop a model of interaction with cellular receptors.